海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
3期
58-60
,共3页
HPLC%士的宁%马钱子碱%颈舒片%含量测定
HPLC%士的寧%馬錢子堿%頸舒片%含量測定
HPLC%사적저%마전자감%경서편%함량측정
HPLC%Strychine%Brucine%Jingshu Tablets%Determination
目的:建立颈舒片中士的宁和马钱子碱的含量测定方法。方法以乙腈-0.01mol· L·-1庚烷磺酸钠与0.02mol· L -1磷酸二氢钾等量混合液(用磷酸调pH至2.6)(19∶81)为流动相,柱温:30℃;检测波长260nm,流速0.9mL· min -1。结果士的宁在11.2~179.2μg· mL -1、马钱子碱在10.6~169.6μg· mL -1浓度范围内线性良好,相关系数分别为 r=0.9999、r =0.9990,回收率分别为98.2%、RSD =0.74%,99.8%、RSD=1.4%。结论本法专属性强,操作简便,可作为腰腿痛片的质量控制方法。
目的:建立頸舒片中士的寧和馬錢子堿的含量測定方法。方法以乙腈-0.01mol· L·-1庚烷磺痠鈉與0.02mol· L -1燐痠二氫鉀等量混閤液(用燐痠調pH至2.6)(19∶81)為流動相,柱溫:30℃;檢測波長260nm,流速0.9mL· min -1。結果士的寧在11.2~179.2μg· mL -1、馬錢子堿在10.6~169.6μg· mL -1濃度範圍內線性良好,相關繫數分彆為 r=0.9999、r =0.9990,迴收率分彆為98.2%、RSD =0.74%,99.8%、RSD=1.4%。結論本法專屬性彊,操作簡便,可作為腰腿痛片的質量控製方法。
목적:건립경서편중사적저화마전자감적함량측정방법。방법이을정-0.01mol· L·-1경완광산납여0.02mol· L -1린산이경갑등량혼합액(용린산조pH지2.6)(19∶81)위류동상,주온:30℃;검측파장260nm,류속0.9mL· min -1。결과사적저재11.2~179.2μg· mL -1、마전자감재10.6~169.6μg· mL -1농도범위내선성량호,상관계수분별위 r=0.9999、r =0.9990,회수솔분별위98.2%、RSD =0.74%,99.8%、RSD=1.4%。결론본법전속성강,조작간편,가작위요퇴통편적질량공제방법。
OBJECTIVE To establish a method for the determination of Strychine and Brucine in Jingshu Tab-lets by HPLC.METHODS The samples were separated on a Hypersil Gold C18(4.6mm ×150mm,5μm)column at the flow rate of 0.9mL· min-1 and the column temperature was 30℃.Acetonitrile and a mixed solution of equal a-mount of 0.01 mol· L-1 Sodium heptanesulfonate and 0.02 mol · L-1 potassium dihydrogen phosphate ( pH was ad-justed to 2.6 by phosphoric acid ) ( 1∶81 ) was used as the mobile phase , the detection wavelength was 240 nm.RESULTS The method had good linear relationship within the range of 11.2 ~179.2μg · mL-1 ( r =0.9999 ) for Strychnine , and 10.6 ~169.6μg · mL-1 ( r=0.9998 ) for Brucine.The average recoveries and RSD were 98.2%and 0.74%( n =9 ) for Strychnine , 99.8%and 1.4%( n =9 ) for Brucine.CONCLUSION The method is specific ,well-done and can be used for the quality control of Jingshu Tablets.