CAJ | 학술논문

目的：通过大鼠海人酸（KA）癫痫模型实验，探讨扩散峰度成像（DKI）应用于原发性癫痫研究的可行性。方法：将KA癫痫模型组（KA组）及正常对照组（NC 组）各20只大鼠，在 GE 3．0T 磁共振仪上行常规 MRI、DKI 及3D-MPRAGE等多序列扫描，在6个部位的灰质（基底节区、海马、杏仁核海马区、颞叶皮层、顶叶皮层、额叶皮层）和3个部位的白质（外囊区、前联合、胼胝体）内选取双侧对称部位的兴趣区（ROI），手动测量各向异性分数（FA）、平均扩散率（MD）、平均峰度值（MK）并进行统计学分析。结果：在KA和NC组间，FA值（双侧均值）差异有统计学意义的部位为杏仁核海马区（分别为0．20±0．04和0．22±0．02，P＝0．0337）、前联合（分别为0．22±0．04和0．28±0．06，P＝0．002）及胼胝体区（分别为0．21±0．05和0．26±0．03，P＝0．0002）；MD值（双侧均值）差异有统计学意义的部位为海马（分别为0．67±0．16和0．74±0．04，P＝0．0275）；MK值（双侧均值）差异有统计学意义的部位为基底节区（分别为0．10±0．26和0．83±0．06， P＝0．0098）、海马（分别为0．83±0．15和0．73±0．09，P＝0．0499）及胼胝体区（分别为0．93±0．22和0．81±0．07，P＝0．036）。结论：扩散峰度成像可用于原发性癫痫的研究，有望成为应用于人类癫痫研究的新的影像学方法。
목적：통과대서해인산（KA）전간모형실험，탐토확산봉도성상（DKI）응용우원발성전간연구적가행성。방법：장KA전간모형조（KA조）급정상대조조（NC 조）각20지대서，재 GE 3．0T 자공진의상행상규 MRI、DKI 급3D-MPRAGE등다서렬소묘，재6개부위적회질（기저절구、해마、행인핵해마구、섭협피층、정협피층、액협피층）화3개부위적백질（외낭구、전연합、변지체）내선취쌍측대칭부위적흥취구（ROI），수동측량각향이성분수（FA）、평균확산솔（MD）、평균봉도치（MK）병진행통계학분석。결과：재KA화NC조간，FA치（쌍측균치）차이유통계학의의적부위위행인핵해마구（분별위0．20±0．04화0．22±0．02，P＝0．0337）、전연합（분별위0．22±0．04화0．28±0．06，P＝0．002）급변지체구（분별위0．21±0．05화0．26±0．03，P＝0．0002）；MD치（쌍측균치）차이유통계학의의적부위위해마（분별위0．67±0．16화0．74±0．04，P＝0．0275）；MK치（쌍측균치）차이유통계학의의적부위위기저절구（분별위0．10±0．26화0．83±0．06， P＝0．0098）、해마（분별위0．83±0．15화0．73±0．09，P＝0．0499）급변지체구（분별위0．93±0．22화0．81±0．07，P＝0．036）。결론：확산봉도성상가용우원발성전간적연구，유망성위응용우인류전간연구적신적영상학방법。
Objective:To study the feasibility of diffusion kurtosis imaging (DKI)in the clinical investigation of pri-mary epilepsy with the experiment of investigating kainic acid (KA)induced epilepsy rats and normal control (NC)rats. Methods:Conventional MRI,DKI and 3D-MPRAGE sequences were used in KA induced rats and NC rats (n= 20/group) with a 3 .0T MR scanner.In six areas of gray matters (GM)including cauduate putamen (CPu),hippocampus (HC),amyg-dalohippocampal area (AHi),temporal cerebral cortex (TCT),parietal cerebral cortex (PCT)and frontal cerebral cortex (FCT)and three areas of white matters (WM)including external capsule (EC),Anterior commissure (AC)and corpus cal-losum (CC),regions of interest (ROI)were selected in symmetric parts of the left and right side.Fractional anisotropy (FA),mean diffusivity (MD),mean kurtosis (MK)values were measured manually and compared,statistic analysis were performed.Results:Of the KA group and NC group of rats,significant differences of FA values (mean FA±SD)were exis-ted in AHi (0.20±0.04、0.22±0.02 respectively,P= 0.0337),AC (0.22±0.04、0.28±0.06 respectively,P= 0.002)and CC area (0.21±0.05、0.26 ±0.03 respectively,P= 0.0002).Significant difference of MD values (mean MD±SD)was existed in HC (0.67±0.16、0.74±0.04 respectively,P= 0.0275).Significant differences of MK values (mean MK±SD) were existed in CPu (0.10±0.26、0.83±0.06 respectively,P= 0.0098),HC (0.83±0.15、0.73±0.09 respectively,P=0.0499)and CC areas (0.93 ±0.22、0.81 ±0.07 respectively,P= 0.0360).Conclusion:The present study demonstrated the successful application of DKI through animal experiment,which propose that DKI sequence could be used as a new method for the research of epilepsy in human beings.