皖南医学院学报
皖南醫學院學報
환남의학원학보
ACTA ACADEMIAE MEDICINAE WANNAN
2013年
4期
259-261
,共3页
罗敏%焦志勇%赵家峰%黄从云
囉敏%焦誌勇%趙傢峰%黃從雲
라민%초지용%조가봉%황종운
人羊水干细胞%培养%鉴定
人羊水榦細胞%培養%鑒定
인양수간세포%배양%감정
human amniotic fluid stem cells%culture%identification
目的：分离培养人羊水干细胞（ hAFSC ）并鉴定。方法：使用贴壁法分离培养羊水干细胞，细胞免疫荧光法和Western blot鉴定hAFSC。结果：分离的羊水干细胞均表达特异性标记物Oct-4、c-kit、SSEA-4、CD105。结论：成功分离及培养hAFSC，可作为种子细胞用于干细胞移植治疗肝纤维化。
目的：分離培養人羊水榦細胞（ hAFSC ）併鑒定。方法：使用貼壁法分離培養羊水榦細胞，細胞免疫熒光法和Western blot鑒定hAFSC。結果：分離的羊水榦細胞均錶達特異性標記物Oct-4、c-kit、SSEA-4、CD105。結論：成功分離及培養hAFSC，可作為種子細胞用于榦細胞移植治療肝纖維化。
목적：분리배양인양수간세포（ hAFSC ）병감정。방법：사용첩벽법분리배양양수간세포，세포면역형광법화Western blot감정hAFSC。결과：분리적양수간세포균표체특이성표기물Oct-4、c-kit、SSEA-4、CD105。결론：성공분리급배양hAFSC，가작위충자세포용우간세포이식치료간섬유화。
Objective:To isolate,culture and identify the stem cells de-rived form human amniotic fluid(hAFSC).Methods:hAFSCs were isola-ted and cultured with adherent method ,and identified by cell immune flu-orescence and Western blotting.Results:Generally,the isolated amniotic stem cells expressed specific markers of Oct-4, c-kit, SSEA-4, and CD105.Conclusion:hAFSC is successfully isolated and cultured,which can be used as the seed cells for management of hepatic fibrosis through stem cell transplantation.
