浙江中医药大学学报
浙江中醫藥大學學報
절강중의약대학학보
JOURNAL OF ZHEJIANG UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
2013年
7期
905-908,909
,共5页
韩淑萍%张卫英%余道军%陈岳明
韓淑萍%張衛英%餘道軍%陳嶽明
한숙평%장위영%여도군%진악명
高效液相色谱法%黄柏%醇提取%水提取%盐酸小檗碱%盐酸药根碱%盐酸巴马汀
高效液相色譜法%黃柏%醇提取%水提取%鹽痠小檗堿%鹽痠藥根堿%鹽痠巴馬汀
고효액상색보법%황백%순제취%수제취%염산소벽감%염산약근감%염산파마정
High Performance Liquid Chromatography%Phel odendri Chinensis Cortex%alcohol extraction%water extraction%Berberine Hydrochloride%Jatrorrhizine Hydrochloride%Palmatine Hydrochloride
[目的]分别测定黄柏盐酸小檗碱、盐酸药根碱、盐酸巴马汀在醇提液和水提液中的含量并进行比较，为课题后续黄柏提取物对抗特定微生物的实验研究提供参考依据。[方法]采用高效液相色谱法，Hypersil BDS C18色谱柱（4.6mm×150mm，5μm），流动相为乙腈-0.1%磷酸溶液（45：55，含0.1%的十二烷基硫酸钠），检测波长为265nm，流速为1mL·min-1，柱温为25℃，分别测定黄柏盐酸小檗碱、盐酸药根碱、盐酸巴马汀在醇提液和水提液中的含量。[结果]在一定浓度范围内盐酸小檗碱、盐酸药根碱、盐酸巴马汀峰面积与浓度均呈良好的线性关系，平均加样回收率分别为101.70%，99.38%，99.06%。相对标准偏差(relative standard devidard,RSD)分别为2.83%，2.21%，1.99%。在醇提取样品中盐酸小檗碱、盐酸药根碱、盐酸巴马汀含量分别是18.5241 mg·mL-1，0.0894mg·mL-1，0.0552 mg·mL-1。在水提取样品中盐酸小檗碱、盐酸药根碱、盐酸巴马汀含量分别是5.7648mg·mL-1,0.0221mg·mL-1，0.0197mg·mL-1。[结论]该方法操作简便、准确、重现性好。黄柏3种生物碱含量在乙醇提取液中均高于水提取液，乙醇提取优于水提取。
[目的]分彆測定黃柏鹽痠小檗堿、鹽痠藥根堿、鹽痠巴馬汀在醇提液和水提液中的含量併進行比較，為課題後續黃柏提取物對抗特定微生物的實驗研究提供參攷依據。[方法]採用高效液相色譜法，Hypersil BDS C18色譜柱（4.6mm×150mm，5μm），流動相為乙腈-0.1%燐痠溶液（45：55，含0.1%的十二烷基硫痠鈉），檢測波長為265nm，流速為1mL·min-1，柱溫為25℃，分彆測定黃柏鹽痠小檗堿、鹽痠藥根堿、鹽痠巴馬汀在醇提液和水提液中的含量。[結果]在一定濃度範圍內鹽痠小檗堿、鹽痠藥根堿、鹽痠巴馬汀峰麵積與濃度均呈良好的線性關繫，平均加樣迴收率分彆為101.70%，99.38%，99.06%。相對標準偏差(relative standard devidard,RSD)分彆為2.83%，2.21%，1.99%。在醇提取樣品中鹽痠小檗堿、鹽痠藥根堿、鹽痠巴馬汀含量分彆是18.5241 mg·mL-1，0.0894mg·mL-1，0.0552 mg·mL-1。在水提取樣品中鹽痠小檗堿、鹽痠藥根堿、鹽痠巴馬汀含量分彆是5.7648mg·mL-1,0.0221mg·mL-1，0.0197mg·mL-1。[結論]該方法操作簡便、準確、重現性好。黃柏3種生物堿含量在乙醇提取液中均高于水提取液，乙醇提取優于水提取。
[목적]분별측정황백염산소벽감、염산약근감、염산파마정재순제액화수제액중적함량병진행비교，위과제후속황백제취물대항특정미생물적실험연구제공삼고의거。[방법]채용고효액상색보법，Hypersil BDS C18색보주（4.6mm×150mm，5μm），류동상위을정-0.1%린산용액（45：55，함0.1%적십이완기류산납），검측파장위265nm，류속위1mL·min-1，주온위25℃，분별측정황백염산소벽감、염산약근감、염산파마정재순제액화수제액중적함량。[결과]재일정농도범위내염산소벽감、염산약근감、염산파마정봉면적여농도균정량호적선성관계，평균가양회수솔분별위101.70%，99.38%，99.06%。상대표준편차(relative standard devidard,RSD)분별위2.83%，2.21%，1.99%。재순제취양품중염산소벽감、염산약근감、염산파마정함량분별시18.5241 mg·mL-1，0.0894mg·mL-1，0.0552 mg·mL-1。재수제취양품중염산소벽감、염산약근감、염산파마정함량분별시5.7648mg·mL-1,0.0221mg·mL-1，0.0197mg·mL-1。[결론]해방법조작간편、준학、중현성호。황백3충생물감함량재을순제취액중균고우수제취액，을순제취우우수제취。
[Objective] Comparison of contents of berberine hydrochloride, jatrorrhizine hydrochloride and palmatine hydrochloride in Phel odendri Chi-nensis Cortex of the alcohol extract and the water extract by HPLC. The experimental study provides reference basis for the subsequent Phel odendri Chinensis Cortex extract against certain microorganisms.[Method] HPLC was performed on Diamonsil Hypersil BDS C18(4.6mm ×150mm, 5μm), with ace-tonitrile-0.1%Phosphoric acid solution(45:55, containing 0.1% twelve sodium alkyl sulfates) as mobile phase. The detective wavelength was set at 265 nm and the column temperature was maintained at 25℃, the gradient elution was carried out with a flow rate of 1.0 mL·min-1. Berberine hydrochloride, jatr-orrhizine hydrochloride and palmatine hydrochloride in Phel odendri Chinensis Cortex of the alcohol extract and the water extract were determined re-spectively.[Result] In a certain concentration range, the peak area of Phel odendri Chinensis Cortex, berberine hydrochloride, jatrorrhizine hydrochloride and palmatine hydrochloride showed a good linear relationship with the concentration. The average recoveries were 101.70%, 99.38%,99.06% and RSD-were 2.83%,2.21%,1.99%respectively . The concents of berberine hydrochloride, jatrorrhizine hydrochloride and palmatine hydrochloride in Phel odendri Chinensis Cortex of the alcohol extract were 18.5241 mg·mL-1,0.0894mg·mL-1 and 0.0552 mg·mL-1 respectively, and which of the water extract were 5.7648mg·mL-1, 0.0221mg·mL-1 and 0.0197mg·mL-1 respectively.[Conclusion]This method was simple, accurate and repeatable. Three alkaloids in Phel-lodendri Chinensis Cortex of the alcohol extract were al higher than of the water extract. The alcohol extraction was superior to the water extraction.
