CAJ | 학술논문

　　[目的]研究缺氧对阴茎海绵体平滑肌细胞(cavernosum smooth muscle cel s ,CCSM）表型转化的影响。[方法]用酶消化法获取SD大鼠的原代CCSM，并用细胞免疫荧光法进行鉴定。将原代CCSM置于缺氧小室中（37℃，5%CO2，1%O2，94%N2）培养，按缺氧时间分为0h组、24h组、48h组、72h组，用western-blot法检测四组的α-SMA、OPN蛋白表达量。[结果]低氧可以诱导CCSMα-SMA蛋白表达下降，且在72h内随着缺氧时间延长差异显著性增加。低氧24h组细胞OPN蛋白表达增加（P<0.05）；低氧48h组细胞OPN蛋白表达显著增加（P<0.01）；48h后随着时间延长差异可能会减小（P>0.05）。[结论]缺氧可以诱导CCSM由收缩型向合成型转化。
　　[목적]연구결양대음경해면체평활기세포(cavernosum smooth muscle cel s ,CCSM）표형전화적영향。[방법]용매소화법획취SD대서적원대CCSM，병용세포면역형광법진행감정。장원대CCSM치우결양소실중（37℃，5%CO2，1%O2，94%N2）배양，안결양시간분위0h조、24h조、48h조、72h조，용western-blot법검측사조적α-SMA、OPN단백표체량。[결과]저양가이유도CCSMα-SMA단백표체하강，차재72h내수착결양시간연장차이현저성증가。저양24h조세포OPN단백표체증가（P<0.05）；저양48h조세포OPN단백표체현저증가（P<0.01）；48h후수착시간연장차이가능회감소（P>0.05）。[결론]결양가이유도CCSM유수축형향합성형전화。
[Objective] To observe the influence of hypoxia on phenotypic modulation in corpus cavernosum smooth muscle cel s(CCSM). [Methods] Pri-mary CCSMC were obtained through enzymatic digestion in SD rat cavernous tissue, identified by immunofluorescence method. Primary CCSM were cultured in hypoxia(37℃, 5%CO2, 1%O2, 94%N2) with different time(0h, 24h, 48h, 72h). Western-blot technique was used to detect the protein expres-sion of α-SMA, OPN in that four groups of hypoxia-induced time. [Results] Hypoxia could down-regulate the protein expression of α-SMA, which was increased markedly with longer anoxia in 72h;the hypoxia in 24h increased the cel OPN protein expression; as wel as in the group of 48h;the dif-ference may decrease after 48h with more time.[Conclusion] Hypoxia can induce the transformation of CCSM from contractile to synthetic.