CAJ | 학술논문

目的：研究丹参对高同型半胱氨酸血症（ HHcy）兔心室重塑的影响。方法：用2％蛋氨酸饮食制备兔HHcy模型，丹参3．3 g/Kg· d灌服干预，以正常家兔为对照，8周末分别检测各组家兔血清丙二醛（MDA）、超氧化物岐化酶（SOD）、同型半胱氨酸（Hcy）、一氧化氮（NO）、内皮素（ET）和血栓素B2（TXB2）含量，处死家兔观察心脏结构病理改变。结果：实验8周时，蛋氨酸组家兔与正常组比较血清Hcy、MDA、ET、TXB2显著升高（P＜0．01），NO、SOD明显降低（P＜0．01）；组织学及免疫组化观察心肌内血管数减少，心肌横径增大，动脉管壁显著增厚，胶原纤维及Actin-平滑肌肌动蛋白表达增强；丹参组家兔与蛋氨酸组相比血清Hcy、MDA、ET、TXB2含量显著降低， NO、SOD水平升高；心肌内血管数目明显回升，胶原纤维和肌动蛋白表达减少。结论：HHcy可诱发兔心室重塑，丹参通过抑制a-SMA的表达、逆转心肌纤维化、提高抗氧化酶的活性等机制，改善和拮抗HHcy 兔的心室重塑作用。
목적：연구단삼대고동형반광안산혈증（ HHcy）토심실중소적영향。방법：용2％단안산음식제비토HHcy모형，단삼3．3 g/Kg· d관복간예，이정상가토위대조，8주말분별검측각조가토혈청병이철（MDA）、초양화물기화매（SOD）、동형반광안산（Hcy）、일양화담（NO）、내피소（ET）화혈전소B2（TXB2）함량，처사가토관찰심장결구병리개변。결과：실험8주시，단안산조가토여정상조비교혈청Hcy、MDA、ET、TXB2현저승고（P＜0．01），NO、SOD명현강저（P＜0．01）；조직학급면역조화관찰심기내혈관수감소，심기횡경증대，동맥관벽현저증후，효원섬유급Actin-평활기기동단백표체증강；단삼조가토여단안산조상비혈청Hcy、MDA、ET、TXB2함량현저강저， NO、SOD수평승고；심기내혈관수목명현회승，효원섬유화기동단백표체감소。결론：HHcy가유발토심실중소，단삼통과억제a-SMA적표체、역전심기섬유화、제고항양화매적활성등궤제，개선화길항HHcy 토적심실중소작용。
Objective:To observe the Effect of Salvia miltiorrhiza on ventricular remodeling in hyperhomocysteinemia ( HHcy) rabbits. Methods:Use 2%methionine (MET) diet to establish the HHcy rabbits models , fed the experimental group with Salvia miltiorrhiza 3. 3g/kg.d for 8 weeks, Detect the content of the malonaldehyde (MDA), the superoxide dismutase (SOD) and the nitric oxide (NO) in each group , observe the pathological changes of cardiac structure at the same time .Results:8 weeks after the experiment , the HCY MDA and TXB2 levels in the MET group were significantly increased (p<0.01), but the level of NO and SOD were significantly de-creased(p<0.01);observe by histology and immunohistochemistry , the number of vessel in myocardium is decreased , the transverse di-ameter of myocardium is increased , the arterial wall is significantly thickened , the express of the collagenous fiber and the smooth muscle actin were enhanced;the MDA and TXB2 levels in the salvia miltiorrhiza group were significantly decreased (p<0.01), but the level of NO and SOD were significantly increased (p<0.01);the number of vascellumin in myocardium is increased , the express of the collage-nous fiber and the smooth muscle actin were decreased .Conclusion:the HHcy can induce the ventricular remodeling of rabbits .because Salvia miltiorrhiza can suppression the express of A -SMA, reverse myocardial fibrosis , increase the antioxidant enzyme activity , it can improve and rival the ventricular remodeling in HHcy rabbits .