海峡药学
海峽藥學
해협약학
STRAIT PHARMACEUTICAL JOURNAL
2014年
3期
30-33
,共4页
熟地黄%大鼠%亚急性毒性
熟地黃%大鼠%亞急性毒性
숙지황%대서%아급성독성
Radix Rehmanniae Preparata%Rat%Subacute toxicity
目的：了解熟地黄植物胶囊的亚急性毒性。方法将大鼠分为阴性对照组及1.688、3.375、6.750g· kg-1 BW 3个剂量组。连续喂养30天后，采血作血常规及生化指标测定，取出肝、肾、脾、睾丸称重，并对肝、肾、脾、胃肠、睾丸、卵巢作组织病理学检查。结果熟地黄植物胶囊各剂量组给予大鼠喂养30天，试验期间，各组动物生长活动正常。试验结束时，各剂量组大鼠体重、体重增重、进食量、食物利用率、脏体比与对照组比较，差异均无统计学意义（P＞0.05）。雄性3.375g· kg -1BW剂量组中性粒细胞，1.688、6.750g· kg-1 BW剂量组血糖，1.688g· kg-1 BW剂量组甘油三酯；雌性3.375、6.750g· kg-1 BW剂量组谷丙转氨酶与对照组相比，差异有统计学意义（P＜0.05），但均在本实验室正常值范围内，本实验室认为无生物学意义。其余各剂量组大鼠的血清谷丙转氨酶、谷草转氨酶、尿素、肌酐、血糖、总胆固醇、甘油三酯、总蛋白、白蛋白测定值、血红蛋白、红细胞计数、白细胞计数和白细胞分类与对照组相比差异均无统计学意义（ P＞0.05）。对受检脏器作组织病理学检查，未见特异性病变。结论大鼠服用熟地黄植物胶囊30天，对机体未见不良影响。
目的：瞭解熟地黃植物膠囊的亞急性毒性。方法將大鼠分為陰性對照組及1.688、3.375、6.750g· kg-1 BW 3箇劑量組。連續餵養30天後，採血作血常規及生化指標測定，取齣肝、腎、脾、睪汍稱重，併對肝、腎、脾、胃腸、睪汍、卵巢作組織病理學檢查。結果熟地黃植物膠囊各劑量組給予大鼠餵養30天，試驗期間，各組動物生長活動正常。試驗結束時，各劑量組大鼠體重、體重增重、進食量、食物利用率、髒體比與對照組比較，差異均無統計學意義（P＞0.05）。雄性3.375g· kg -1BW劑量組中性粒細胞，1.688、6.750g· kg-1 BW劑量組血糖，1.688g· kg-1 BW劑量組甘油三酯；雌性3.375、6.750g· kg-1 BW劑量組穀丙轉氨酶與對照組相比，差異有統計學意義（P＜0.05），但均在本實驗室正常值範圍內，本實驗室認為無生物學意義。其餘各劑量組大鼠的血清穀丙轉氨酶、穀草轉氨酶、尿素、肌酐、血糖、總膽固醇、甘油三酯、總蛋白、白蛋白測定值、血紅蛋白、紅細胞計數、白細胞計數和白細胞分類與對照組相比差異均無統計學意義（ P＞0.05）。對受檢髒器作組織病理學檢查，未見特異性病變。結論大鼠服用熟地黃植物膠囊30天，對機體未見不良影響。
목적：료해숙지황식물효낭적아급성독성。방법장대서분위음성대조조급1.688、3.375、6.750g· kg-1 BW 3개제량조。련속위양30천후，채혈작혈상규급생화지표측정，취출간、신、비、고환칭중，병대간、신、비、위장、고환、란소작조직병이학검사。결과숙지황식물효낭각제량조급여대서위양30천，시험기간，각조동물생장활동정상。시험결속시，각제량조대서체중、체중증중、진식량、식물이용솔、장체비여대조조비교，차이균무통계학의의（P＞0.05）。웅성3.375g· kg -1BW제량조중성립세포，1.688、6.750g· kg-1 BW제량조혈당，1.688g· kg-1 BW제량조감유삼지；자성3.375、6.750g· kg-1 BW제량조곡병전안매여대조조상비，차이유통계학의의（P＜0.05），단균재본실험실정상치범위내，본실험실인위무생물학의의。기여각제량조대서적혈청곡병전안매、곡초전안매、뇨소、기항、혈당、총담고순、감유삼지、총단백、백단백측정치、혈홍단백、홍세포계수、백세포계수화백세포분류여대조조상비차이균무통계학의의（ P＞0.05）。대수검장기작조직병이학검사，미견특이성병변。결론대서복용숙지황식물효낭30천，대궤체미견불량영향。
OBJECTIVE To explore the subacute toxicity of Radix Rehmanniae Preparata integrated plant cap-sules.METHODS Rats were randomly olivided into 4 different categories which included 1.688 g · kg -1、3.375 g · kg -1、6.750 g· kg -1 and control group.routine test and biochemical indexes of blood were determinated after 30 days of continuous feeding,liver,kidney,spleen and testes were weighed,histopathological examination were per-formed on the liver ,kidney ,spleen ,stomach ,ovary and testis.RESULTS During the period of experiment ,the nor-mal growth activites of rats in different group were observed.At the end of the experiment ,difference between differ-ent group on the body weight ,increasing weight ,food intake ,food utilization rate ,and organ/body weight ratio of rats were not statistically significant (P>0.05).Statistically significance were found in the neutrophils of male 3.375g· kg -1 group , the blood sugar of 1.688 g · kg -1、6.750 g · kg -1 group , the triglyceride of 1.688 g · kg -1 group.The difference of alanine aminotransferase between 3.375 g· kg -1、6.750 g· kg-1 and control group were also statistically significant ( P<0.05 ) and within the normal range of this laboratory ,which indicated no biological significance.No statistical significance were observed among the other group on the serum alanine aminotransferase ,aspartate amin-otransferase,urea,creatinine,blood glucose,total cholesterol,triglyceride,total protein,albumin,hemoglobin determi-nation value ,red blood cell count ,white blood cell count and classification.Histopathological examination showed no specific lesions.CONCLUSION The study demonstrates that there is no adverse effects to rat after 30 days of con-tinuous feeding of Radix rehmanniae preparata integrated plant capsules.
