光谱学与光谱分析
光譜學與光譜分析
광보학여광보분석
SPECTROSCOPY AND SPECTRAL ANALYSIS
2014年
4期
1040-1044
,共5页
李月生%杜纪富%孙绍发%赵龙
李月生%杜紀富%孫紹髮%趙龍
리월생%두기부%손소발%조룡
共振光散射法%外源刺激%CdSe/ZnS量子点/TiO2%人血清白蛋白%相互作用
共振光散射法%外源刺激%CdSe/ZnS量子點/TiO2%人血清白蛋白%相互作用
공진광산사법%외원자격%CdSe/ZnS양자점/TiO2%인혈청백단백%상호작용
Resonance light scattering (RLS)%Exogenous stimulus%CdSe/ZnS(quantum dots)/TiO2%Human serum albumin%Interaction
近似生理条件下,采用共振光散射法研究了CdSe/ZnS量子点/TiO2纳米复合物同人血清白蛋白的相互作用,通过分析影响二者相互作用的纳米复合物浓度、pH、NaCl浓度、反应温度、检测时间、共存离子、表面活性剂、加样顺序等外源刺激因素,结果表明:新形成的复合体系可能加强蛋白质的疏水腔,使在水溶液中形成的疏水界面趋于集中,从而导致其共振光散射强度增强;体系受pH的影响变化非常灵敏;适当的NaCl浓度可以提高体系的 IRLS值灵敏度;共存离子改变了体系的离子强度,从而导致体系的ΔIRLS值的改变;反应时间为5 min时,体系 IRLS值基本稳定;同一种表面活性剂对于不同的体系的 IRLS值的作用不完全一致,带相反电荷的表面活性剂与纳米复合物有很强的静电作用;对于与 HSA相互作用的多元复合体系,加样顺序的不同对体系的 IRLS值的影响很明显;体系的 IRLS值随温度的改变呈不完全单调增加的趋势。这些信息为纳米材料与生物大分子的相互作用机制提供理论支撑,有助于深入了解纳米材料的生物相容性和安全性。
近似生理條件下,採用共振光散射法研究瞭CdSe/ZnS量子點/TiO2納米複閤物同人血清白蛋白的相互作用,通過分析影響二者相互作用的納米複閤物濃度、pH、NaCl濃度、反應溫度、檢測時間、共存離子、錶麵活性劑、加樣順序等外源刺激因素,結果錶明:新形成的複閤體繫可能加彊蛋白質的疏水腔,使在水溶液中形成的疏水界麵趨于集中,從而導緻其共振光散射彊度增彊;體繫受pH的影響變化非常靈敏;適噹的NaCl濃度可以提高體繫的 IRLS值靈敏度;共存離子改變瞭體繫的離子彊度,從而導緻體繫的ΔIRLS值的改變;反應時間為5 min時,體繫 IRLS值基本穩定;同一種錶麵活性劑對于不同的體繫的 IRLS值的作用不完全一緻,帶相反電荷的錶麵活性劑與納米複閤物有很彊的靜電作用;對于與 HSA相互作用的多元複閤體繫,加樣順序的不同對體繫的 IRLS值的影響很明顯;體繫的 IRLS值隨溫度的改變呈不完全單調增加的趨勢。這些信息為納米材料與生物大分子的相互作用機製提供理論支撐,有助于深入瞭解納米材料的生物相容性和安全性。
근사생리조건하,채용공진광산사법연구료CdSe/ZnS양자점/TiO2납미복합물동인혈청백단백적상호작용,통과분석영향이자상호작용적납미복합물농도、pH、NaCl농도、반응온도、검측시간、공존리자、표면활성제、가양순서등외원자격인소,결과표명:신형성적복합체계가능가강단백질적소수강,사재수용액중형성적소수계면추우집중,종이도치기공진광산사강도증강;체계수pH적영향변화비상령민;괄당적NaCl농도가이제고체계적 IRLS치령민도;공존리자개변료체계적리자강도,종이도치체계적ΔIRLS치적개변;반응시간위5 min시,체계 IRLS치기본은정;동일충표면활성제대우불동적체계적 IRLS치적작용불완전일치,대상반전하적표면활성제여납미복합물유흔강적정전작용;대우여 HSA상호작용적다원복합체계,가양순서적불동대체계적 IRLS치적영향흔명현;체계적 IRLS치수온도적개변정불완전단조증가적추세。저사신식위납미재료여생물대분자적상호작용궤제제공이론지탱,유조우심입료해납미재료적생물상용성화안전성。
The interaction between CdSe/ZnS(quantum dots)/TiO2 nanocomposites and human serum albumin(HSA) was inves-tigated by resonance light scattering (RLS) spectroscopic methods under approximate physiological conditions .Much important information of the interaction between CdSe/ZnS(Quantum Dots)/TiO2 nanocomposites and HSA was obtained by studying com-prehensively the Exogenous influence factors of nanocomposites concentration ,pH ,NaCl concentration ,reaction temperature , detection time ,coexisting ions ,surfactants ,sequence of adding to the sample etc .It was showed that the new formation of com-plex system is likely to enhance the protein hydrophobic cavity and tend to focus the hydrophobic interface in aqueous solution , resulting in strengthening the intensity of resonance light scattering ;Also it is very sensitive to the changes in the pH value in the system ;The sensitivity of IRLS in system can be increased by the appropriate NaCl concentration ;The value of IRLS in sys-tem would be changed with the change in the concentration of coexisting ions ;The value of IRLS in system is basically stable when the reaction time reaches 5 min;The value of IRLS in system is not exactly the same with a surfactant ,and strong electrostatic in-teraction has occurred between oppositely charged surfactant and nano composites ;It is obvious that the value of IRLS in complex system is affected by the sequence of adding to sample ;It has the incomplete monotonically increasing trend with the changes in temperature .The information is useful for providing theoretical supporting for the mechanism of interaction between nanomateri-als and bio-macromolecule ,and for understanding the biocompatibility and safety of nano-materials .