CAJ | 학술논문

研究了二氢卟吩e6-C15单甲酯在不同溶剂体系中的荧光光谱特性，据此建立了检测血浆中二氢卟吩e6-C15单甲酯的荧光分析法。首先，通过比较分析二氢卟吩e6-C15单甲酯在甲醇、乙醇、丙酮、乙腈、磷酸盐缓冲液和水六种不同溶剂体系中的荧光光谱特征，考察了溶剂体系对二氢卟吩e6-C15单甲酯荧光光谱特性的影响，结果发现甲醇和乙腈是比较理想的溶剂体系，而磷酸盐缓冲液优于水溶液。通过进一步考察有机溶剂含量和溶液p H对二氢卟吩e6-C15单甲酯发射波长和荧光强度的影响，结果表明p H 7.2磷酸盐缓冲液-乙腈（3∶7）混合溶液中二氢卟吩e6-C15单甲酯荧光强度大、性质稳定。采用p H 7.2磷酸盐缓冲液-乙腈（3∶7）混合溶液沉淀SD大鼠血浆蛋白，选择498.00和664.05 nm作为激发波长和发射波长，建立的SD大鼠血浆样品中二氢卟吩e6-C15单甲酯荧光分析法专属性好，灵敏度高，线性范围为0.50～50.00μg · mL -1（R2≥0.999），批内和批间精密度RSD均小于10％，提取回收率大于90％。该方法简便、快速、可靠，成功地应用于二氢卟吩e6-C15单甲酯在SD大鼠体内的药代动力学研究。
연구료이경계분e6-C15단갑지재불동용제체계중적형광광보특성，거차건립료검측혈장중이경계분e6-C15단갑지적형광분석법。수선，통과비교분석이경계분e6-C15단갑지재갑순、을순、병동、을정、린산염완충액화수륙충불동용제체계중적형광광보특정，고찰료용제체계대이경계분e6-C15단갑지형광광보특성적영향，결과발현갑순화을정시비교이상적용제체계，이린산염완충액우우수용액。통과진일보고찰유궤용제함량화용액p H대이경계분e6-C15단갑지발사파장화형광강도적영향，결과표명p H 7.2린산염완충액-을정（3∶7）혼합용액중이경계분e6-C15단갑지형광강도대、성질은정。채용p H 7.2린산염완충액-을정（3∶7）혼합용액침정SD대서혈장단백，선택498.00화664.05 nm작위격발파장화발사파장，건립적SD대서혈장양품중이경계분e6-C15단갑지형광분석법전속성호，령민도고，선성범위위0.50～50.00μg · mL -1（R2≥0.999），비내화비간정밀도RSD균소우10％，제취회수솔대우90％。해방법간편、쾌속、가고，성공지응용우이경계분e6-C15단갑지재SD대서체내적약대동역학연구。
This paper studied the fluorescence spectral characteristic of chlorin e6-C15-monomethyl ester in different solvents to develop a fluorescence spectrometry for determining the concentration of chlorin e6-C15-monomethyl ester in plasma .By compa-ring the fluorescence spectral characteristics of chlorin e6-C15-monomethyl ester in six different solvents including methanol ,eth-anol ,acetone ,acetonitrile ,phosphate buffered saline and water ,the influence of different solvents on the fluorescence spectral characteristic of chlorin e6-C15-monomethyl ester has been examined .The results indicated that methanol and acetonitrile are the ideal solvent system ,and the effect of phosphate buffered saline is better than water .The effect of organic solvent content and solution pH value on emission wavelength and fluorescence intensity was further evaluated .It was found that the fluorescence in-tensity of chlorin e6-C15-monomethyl ester was strong and stable in pH 7.2 phosphate buffered saline-acetonitrile (3∶7) solu-tion and can be detected at the excitation wavelength of 498.00 nm and the emission wavelength of 664.05 nm .Based on this ,we developed the fluorescence spectrometry for determining chlorin e6-C15-monomethyl ester .It is specific and sensitive with good linearity over the range of 0.5~50 μg · mL -1 .The intra-batch and inter-batch precisions (RSD) were less than 10% ,the ex-traction recoveries were all over 90% .The established method in this paper was simple ,fast ,effective and could be successfully applied to study the pharmacokinetics of chlorine e6-C15-monomethyl ester in SD rats .