CAJ | 학술논문

建立了薄层色谱（TLC）与表面增强拉曼光谱（SERS）联用方法对降压类中药中四种非法添加的化学成分，即盐酸尼卡地平、甲磺酸多沙唑嗪、盐酸普萘洛尔及氢氯噻嗪进行快速检测的新方法。利用 T LC法将掺杂成分与中药基质进行简单分离，采用SERS法对薄层板上的微量物质进行定性检测。实验中通过优化银胶浓度和薄层展开体系，并考察掺杂成分的最低检测限，初步建立可用于降压类中药非法添加化学成分的TLC-SERS检测方法。结果表明，该方法用于检测这四种化学成分时专属性较好，检测限低可至0.005μg，最终检测出十种降压类中药中有两种非法添加了化学成分。本方法简便快速，能实现降压类中药中非法添加化学成分的快速检测，有望成为中药掺杂化学成分的现场检测的新方法。
건립료박층색보（TLC）여표면증강랍만광보（SERS）련용방법대강압류중약중사충비법첨가적화학성분，즉염산니잡지평、갑광산다사서진、염산보내락이급경록새진진행쾌속검측적신방법。이용 T LC법장참잡성분여중약기질진행간단분리，채용SERS법대박층판상적미량물질진행정성검측。실험중통과우화은효농도화박층전개체계，병고찰참잡성분적최저검측한，초보건립가용우강압류중약비법첨가화학성분적TLC-SERS검측방법。결과표명，해방법용우검측저사충화학성분시전속성교호，검측한저가지0.005μg，최종검측출십충강압류중약중유량충비법첨가료화학성분。본방법간편쾌속，능실현강압류중약중비법첨가화학성분적쾌속검측，유망성위중약참잡화학성분적현장검측적신방법。
A novel facile method for on-site detection of antipertensive chemicals (e .g .nicardipine hydrochloride ,doxazosin me-sylate ,propranolol hydrochloride ,and hydrochlorothiazide) adulterated in traditional Chinese medicine for hypertension using thin layer chromatography (TLC) combined with surface enhanced Raman spectroscopy (SERS) was reported in the present pa-per .Analytes and pharmaceutical matrices was separated by TLC ,then SERS method was used to complete qualitative identifi-cation of trace substances on TLC plate .By optimizing colloidal silver concentration and developing solvent ,as well as exploring the optimal limits of detection (LOD) ,the initially established TLC-SERS method was used to detect real hypertension Chinese pharmaceuticals .The results showed that this method had good specificity for the four chemicals and high sensitivity with a limit of detection as lower as to 0.005μg .Finally ,two of the ten antipertensive drugs were detected to be adulterated with chemicals . This simple and fast method can realize rapid detection of chemicals illegally for doping in antipertensive Chinese pharmaceuti-cals ,and would have good prospects in on-site detection of chemicals for doping in Chinese pharmaceuticals .