CAJ | 학술논문

利用傅里叶红外光谱（FTIR）研究三种烟草品种DNA的差异，旨在对烟草品种进行亲缘关系分析和品种鉴定。研究结果显示，三种烟草品种DNA 红外光谱较为相似，均有四个明显的特征峰，1103 cm-1是DNA磷酸二酯键的对称伸缩振动，1236 cm -1是DNA 磷酸二酯键的非对称伸缩振动，1400 cm -1是DNA糖苷键的伸缩振动，1622 cm -1是DNA中是胞嘧啶C4-C5＝C6的环伸缩振动。通过平滑、标准化处理、二阶求导、提取主成分和系统聚类分析建立了DNA FTIR光谱数据聚类分析模型。使用该模型对三个烟草品种进行鉴定，鉴定正确率为100％。使用该模型对三个烟草品种进行亲缘关系分析，云烟87与K326聚为一类，距离系数为0.003，DNA相似度为99.7％，红大单独聚为一类。聚类正确率达100％。该项研究为烟草品种鉴定及遗传育种提供了参考。
이용부리협홍외광보（FTIR）연구삼충연초품충DNA적차이，지재대연초품충진행친연관계분석화품충감정。연구결과현시，삼충연초품충DNA 홍외광보교위상사，균유사개명현적특정봉，1103 cm-1시DNA린산이지건적대칭신축진동，1236 cm -1시DNA 린산이지건적비대칭신축진동，1400 cm -1시DNA당감건적신축진동，1622 cm -1시DNA중시포밀정C4-C5＝C6적배신축진동。통과평활、표준화처리、이계구도、제취주성분화계통취류분석건립료DNA FTIR광보수거취류분석모형。사용해모형대삼개연초품충진행감정，감정정학솔위100％。사용해모형대삼개연초품충진행친연관계분석，운연87여K326취위일류，거리계수위0.003，DNA상사도위99.7％，홍대단독취위일류。취류정학솔체100％。해항연구위연초품충감정급유전육충제공료삼고。
The aim is analyzing genetic reLationship and identifying varieties by detecting DNA differences of three kinds of Nic-otiana tabacum L .using Fourier infrared spectrum (FTIR) .Results show that DNA FTIR of three kinds of Nicotiana tabacum L .is relatively similar .They all have four obvious characteristic peaks .1 105 cm -1 beLongs to symmetrical stretching vibration of phosphodiester bond ,1 250 cm -1 is unsymmetrical stretching vibration of phosphodiester bond ,1 400 cm-1 is contributed to glucosidic bond ,and 1 622 cm-1 belongs to C4 C5 C6 stretching vibration of cytosine .DNA FTIR data was handled by smoothing ,standardizing ,second derivative ,principal component analysis and Hierarchical cluster analysis .The standard model of Hierarchical cluster combined with principal component of the second derivative was set up .The correct rate of identification is 100% .Yunyan 87 and K326 were clustered into one by using the model .The distance coefficient is 0.003 ,and DNA similari-ty is 99.7% ,Hongda was clustered into one by itself .The correct rate of cluster is 100% .The study provides a reference for N icotiana tabacum L .variety identification and genetic breeding .