国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2013年
11期
825-829,封3
,共6页
慢性阻塞性肺疾病%肺功能%大鼠
慢性阻塞性肺疾病%肺功能%大鼠
만성조새성폐질병%폐공능%대서
Chronic obstructive pulmonary disease%Spirometry%Rat
目的 动态观察不同烟熏时间建立的慢性阻塞性肺疾病(COPD)大鼠肺功能和病理的改变,为成功建立COPD大鼠模型提供理论基础.方法 30只8周龄SD大鼠随机分为6周实验组(E1组)及6周对照组(C1组),12周实验组(E2组)及12周对照组(C2组),间隔实验组(烟熏12周后正常饲养6周,E3组).Buxco小动物肺功能仪检测肺功能、HE染色观察肺组织病理变化.结果 ①与C1组相比,E1组FRC、IC、VC和TLC增高(P＜0.05),FEV100/FVC和FEV200/FVC分别降低22％和11％(P＜0.01)；②与C2组相比,E2组FRC增高,IC、VC和TLC降低(P＜0.05),FEV100/FVC和FEV200/FVC分别降低28％和21％(P＜0.01)；③与C2组相比,E3组IC、VC和TLC升高(P＜0.05),FEV100/FVC和FEV200/FVC差异无统计学意义；④E1组肺泡间隔变窄,呈轻度肺气肿表现,局部可见炎症细胞浸润；E2组呈典型肺气肿表现,细支气管、血管周围有大量炎症细胞浸润；E3组肺气肿表现明显,但未见明显炎症；C1、C2组气道上皮结构完整,肺泡间隔完整.结论 烟熏时间对大鼠肺功能和病理改变的程度有显著影响,烟熏6周出现早期COPD表现,烟熏12周出现典型COPD表现.
目的 動態觀察不同煙熏時間建立的慢性阻塞性肺疾病(COPD)大鼠肺功能和病理的改變,為成功建立COPD大鼠模型提供理論基礎.方法 30隻8週齡SD大鼠隨機分為6週實驗組(E1組)及6週對照組(C1組),12週實驗組(E2組)及12週對照組(C2組),間隔實驗組(煙熏12週後正常飼養6週,E3組).Buxco小動物肺功能儀檢測肺功能、HE染色觀察肺組織病理變化.結果 ①與C1組相比,E1組FRC、IC、VC和TLC增高(P＜0.05),FEV100/FVC和FEV200/FVC分彆降低22％和11％(P＜0.01)；②與C2組相比,E2組FRC增高,IC、VC和TLC降低(P＜0.05),FEV100/FVC和FEV200/FVC分彆降低28％和21％(P＜0.01)；③與C2組相比,E3組IC、VC和TLC升高(P＜0.05),FEV100/FVC和FEV200/FVC差異無統計學意義；④E1組肺泡間隔變窄,呈輕度肺氣腫錶現,跼部可見炎癥細胞浸潤；E2組呈典型肺氣腫錶現,細支氣管、血管週圍有大量炎癥細胞浸潤；E3組肺氣腫錶現明顯,但未見明顯炎癥；C1、C2組氣道上皮結構完整,肺泡間隔完整.結論 煙熏時間對大鼠肺功能和病理改變的程度有顯著影響,煙熏6週齣現早期COPD錶現,煙熏12週齣現典型COPD錶現.
목적 동태관찰불동연훈시간건립적만성조새성폐질병(COPD)대서폐공능화병리적개변,위성공건립COPD대서모형제공이론기출.방법 30지8주령SD대서수궤분위6주실험조(E1조)급6주대조조(C1조),12주실험조(E2조)급12주대조조(C2조),간격실험조(연훈12주후정상사양6주,E3조).Buxco소동물폐공능의검측폐공능、HE염색관찰폐조직병리변화.결과 ①여C1조상비,E1조FRC、IC、VC화TLC증고(P＜0.05),FEV100/FVC화FEV200/FVC분별강저22％화11％(P＜0.01)；②여C2조상비,E2조FRC증고,IC、VC화TLC강저(P＜0.05),FEV100/FVC화FEV200/FVC분별강저28％화21％(P＜0.01)；③여C2조상비,E3조IC、VC화TLC승고(P＜0.05),FEV100/FVC화FEV200/FVC차이무통계학의의；④E1조폐포간격변착,정경도폐기종표현,국부가견염증세포침윤；E2조정전형폐기종표현,세지기관、혈관주위유대량염증세포침윤；E3조폐기종표현명현,단미견명현염증；C1、C2조기도상피결구완정,폐포간격완정.결론 연훈시간대대서폐공능화병리개변적정도유현저영향,연훈6주출현조기COPD표현,연훈12주출현전형COPD표현.
Objective To observe the dynamic changes in spirometry and pathology of smokeinduced chronic obstructive pulmonary disease (COPD) rat,and provide a theoretical basis for the successful establishment of COPD rat model.Methods Thirty SD rats at 8 weeks of age were randomized divided into 6 weeks smoke-exposed group (E1 group) and 6 week sham-exposed group (C1 group),12weeks smoke-exposed group (E2 group) and 12 weeks sham-exposed group (C2 group),interval smokeexpose group (normal feeding 6 weeks after smoke for 12 weeks,E3 group).Using Buxco research system to obtain the spirometry data and HE staining to observe the pathological changes of lung tissue.Results ①Compared with C1 group,FRC,IC,VC and TLC of E1 group were increased (P ＜0.05),FEV100/FVC,FEV200/FVC were reduced by 22％,11％ respectively (P ＜0.01).②Compared with C2 group,the FRC of E2 group was increased while IC,VC,TLC were decreased (P ＜0.05),FEV100/FVC and FEV200/FVC were reduced by 28％ and 21％ (P ＜0.01).③Compared with E2 group,IC,VC,TLC of E3 group were increased (P ＜ 0.05),while no difference in FEV100/FVC,FEV200/FVC.④ The pathological examination of lungs from E1 group displayed narrowed alveolar septa which was the performance of mild emphysema,and with partial infiltration of characteristic inflammatory cells.The alveolar of E2 group displayed typical performance of emphysema with infiltration of a large number of inflammatory cell surrouding bronchioles and vesseles.E3 group displayed obvious emphysema while no significant inflammation was detected.C1 and C2 group showed the structural intergity of airway epithelium and alveolar septa.Conclusions Smoking time affects the spirometry and pathology of smoke induced rat typically.With 6 weeks smoking time,the rats performed early-stage COPD while with 12 weeks smoking time performed typical COPD.
