现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2014年
6期
104-104
,共1页
丁芳%侯倩倩%刘广聊%孙喜云%孙凤岭
丁芳%侯倩倩%劉廣聊%孫喜雲%孫鳳嶺
정방%후천천%류엄료%손희운%손봉령
怀地黄%组织培养%快繁%培养基
懷地黃%組織培養%快繁%培養基
부지황%조직배양%쾌번%배양기
Rehmannia glutinosa Libosch%tissue culture%rapid propagation%culture medium
研究怀地黄组织培养与快繁技术。以怀地黄茎尖部分为外植体，在以MS作为基本培养基的基础上，添加不同浓度的NAA、IAA、2，4-D、6-BA，配制不同浓度的培养基，对怀地黄的茎尖进行分化与诱导、继代与增殖及生根培养试验，并对试管苗进行炼苗移栽。获得了怀地黄茎尖培养脱毒与诱导、增殖和生根的培养基，以及生长所需要的外部条件和配套技术。
研究懷地黃組織培養與快繁技術。以懷地黃莖尖部分為外植體，在以MS作為基本培養基的基礎上，添加不同濃度的NAA、IAA、2，4-D、6-BA，配製不同濃度的培養基，對懷地黃的莖尖進行分化與誘導、繼代與增殖及生根培養試驗，併對試管苗進行煉苗移栽。穫得瞭懷地黃莖尖培養脫毒與誘導、增殖和生根的培養基，以及生長所需要的外部條件和配套技術。
연구부지황조직배양여쾌번기술。이부지황경첨부분위외식체，재이MS작위기본배양기적기출상，첨가불동농도적NAA、IAA、2，4-D、6-BA，배제불동농도적배양기，대부지황적경첨진행분화여유도、계대여증식급생근배양시험，병대시관묘진행련묘이재。획득료부지황경첨배양탈독여유도、증식화생근적배양기，이급생장소수요적외부조건화배투기술。
The paper aimed to study thevirus-free tissue culture and the rapid propagation technology.With Rehmannia glutinosa Libosch shoot tip as explants,MS was used as the basic medium with different concentration of NAA,IAA,2,4-D and 6-BA to configure different media,the tests of differentiation and induction,subculture and proliferation as well as rooting culture on Rehmannia glutinosa Libosch were carried out and the test-tube plantlets were hardened and transplanted.In the experiment,the media of detoxification and induction,proliferation and rooting were obtained as well as the external environmental conditions for the plant growth and the matching technology.
