广州化工
廣州化工
엄주화공
GUANGZHOU CHEMICAL INDUSTRY AND TECHNOLOGY
2012年
9期
111-113
,共3页
氧化亚铁硫杆菌%黄钾铁矾沉淀%减少%优化培养基
氧化亞鐵硫桿菌%黃鉀鐵礬沉澱%減少%優化培養基
양화아철류간균%황갑철반침정%감소%우화배양기
acidithiobacillus ferrooxidans%jarosite deposition%reduction%optimization culture
为了减少氧化亚铁硫杆菌培养过程中黄钾铁矾沉淀的生成，通过改变培养基的pH值、菌种的培养温度、不同的菌种接种量及优化培养基组分等方法，确定出黄钾铁矾沉淀生成量最少的培养条件，即培养基pH值为2．0，培养温度为30℃，菌种接种量为10％，最佳培养基组分K2HPO4为0．25g/L，KH2PO4为0．195g／L。此条件下即能保持菌种的良好活性，又能使产生沉淀量降至2．27g／L。
為瞭減少氧化亞鐵硫桿菌培養過程中黃鉀鐵礬沉澱的生成，通過改變培養基的pH值、菌種的培養溫度、不同的菌種接種量及優化培養基組分等方法，確定齣黃鉀鐵礬沉澱生成量最少的培養條件，即培養基pH值為2．0，培養溫度為30℃，菌種接種量為10％，最佳培養基組分K2HPO4為0．25g/L，KH2PO4為0．195g／L。此條件下即能保持菌種的良好活性，又能使產生沉澱量降至2．27g／L。
위료감소양화아철류간균배양과정중황갑철반침정적생성，통과개변배양기적pH치、균충적배양온도、불동적균충접충량급우화배양기조분등방법，학정출황갑철반침정생성량최소적배양조건，즉배양기pH치위2．0，배양온도위30℃，균충접충량위10％，최가배양기조분K2HPO4위0．25g/L，KH2PO4위0．195g／L。차조건하즉능보지균충적량호활성，우능사산생침정량강지2．27g／L。
In order to reduce jarosite precipitate generated in the growth process of acidithiobacillus ferrooxidans, b changing Y the pH value of the culture, the cuhivation temperature, different inoculation quantity and optimization culture, composition method, the conditions with the least amount of jarosite precipitation production were determined that the medium pH value was 2.0, temperature was 30℃, inoculation quantity was 10%, the best component medium was K2HPO4 0.25 g/L, and KH2PO4 0. 195 g/L. The conditions can maintain activity of the bacteria, and can make the precipitation amount down to 2.27 g/L.
