体育科技文献通报
體育科技文獻通報
체육과기문헌통보
BULLETIN OF SPORT SCIENCE & TECHNOLOGY
2012年
7期
3-5,8
,共4页
方亮%刘庆山%王维群%段云霞%陈小玉%张梓倩
方亮%劉慶山%王維群%段雲霞%陳小玉%張梓倩
방량%류경산%왕유군%단운하%진소옥%장재천
强制性运动疗法%脑动脉%缺血%神经血管%细胞
彊製性運動療法%腦動脈%缺血%神經血管%細胞
강제성운동요법%뇌동맥%결혈%신경혈관%세포
constraint- induced movement therapy%effective compounds group of 'Baimai Powder'%middle cerebral artery occlusion reperfusion models%neurovascular unit%cell apoptosis
目的：研究强制性运动疗法、“白脉散”抗脑缺血有效成分纽及二者联用对大鼠脑缺血再灌注损伤的保护作用及相关机制。方法：75只雄性SD大鼠随机分为假手术组（Sham）、模型组（MCAO／R）、银杏叶提取物组（EGB）、强制性运动疗法组（CIMT）、“白脉散”有效成分组（ECG），强制性运动疗法＋“白脉散”有效成分组（CIMT＋ECG），每组7只。采用线栓法制作大鼠大脑中动脉闭塞再灌注模型，缺血2h再灌注24h后进行神经功能评分，然后ECG组及cIMT＋ECG组以155mg／kg／d灌胃，EGB组以5．6mg／kg／d灌胃，其余组灌胃等体积的溶媒，同时CIMT组及CIMT＋ECG组建立运动疗法模型，每天1次，连续7天。利用免疫组化法观察神经血管单元（NVU）细胞中Bcl-2、Caspase-3蛋白表达的情况，运用原位末端标记（TUNEL）法计数NVU凋亡细胞。结果：CIMT与“白脉散”ECG单独或联合应用可显著改善模型大鼠神经功能评分、抑制NVU细胞凋亡、增加Bcl-2蛋白的表达，降低Caspase-3蛋白的表达。结论：CIMT及“白脉散”ECG可保护MCAO／R模型大鼠神经功能，其机制可能与其抗NVU细胞凋亡，调节凋亡调控因子蛋白表达有关，且二者联用比二者单用具有更佳的保护效果。
目的：研究彊製性運動療法、“白脈散”抗腦缺血有效成分紐及二者聯用對大鼠腦缺血再灌註損傷的保護作用及相關機製。方法：75隻雄性SD大鼠隨機分為假手術組（Sham）、模型組（MCAO／R）、銀杏葉提取物組（EGB）、彊製性運動療法組（CIMT）、“白脈散”有效成分組（ECG），彊製性運動療法＋“白脈散”有效成分組（CIMT＋ECG），每組7隻。採用線栓法製作大鼠大腦中動脈閉塞再灌註模型，缺血2h再灌註24h後進行神經功能評分，然後ECG組及cIMT＋ECG組以155mg／kg／d灌胃，EGB組以5．6mg／kg／d灌胃，其餘組灌胃等體積的溶媒，同時CIMT組及CIMT＋ECG組建立運動療法模型，每天1次，連續7天。利用免疫組化法觀察神經血管單元（NVU）細胞中Bcl-2、Caspase-3蛋白錶達的情況，運用原位末耑標記（TUNEL）法計數NVU凋亡細胞。結果：CIMT與“白脈散”ECG單獨或聯閤應用可顯著改善模型大鼠神經功能評分、抑製NVU細胞凋亡、增加Bcl-2蛋白的錶達，降低Caspase-3蛋白的錶達。結論：CIMT及“白脈散”ECG可保護MCAO／R模型大鼠神經功能，其機製可能與其抗NVU細胞凋亡，調節凋亡調控因子蛋白錶達有關，且二者聯用比二者單用具有更佳的保護效果。
목적：연구강제성운동요법、“백맥산”항뇌결혈유효성분뉴급이자련용대대서뇌결혈재관주손상적보호작용급상관궤제。방법：75지웅성SD대서수궤분위가수술조（Sham）、모형조（MCAO／R）、은행협제취물조（EGB）、강제성운동요법조（CIMT）、“백맥산”유효성분조（ECG），강제성운동요법＋“백맥산”유효성분조（CIMT＋ECG），매조7지。채용선전법제작대서대뇌중동맥폐새재관주모형，결혈2h재관주24h후진행신경공능평분，연후ECG조급cIMT＋ECG조이155mg／kg／d관위，EGB조이5．6mg／kg／d관위，기여조관위등체적적용매，동시CIMT조급CIMT＋ECG조건립운동요법모형，매천1차，련속7천。이용면역조화법관찰신경혈관단원（NVU）세포중Bcl-2、Caspase-3단백표체적정황，운용원위말단표기（TUNEL）법계수NVU조망세포。결과：CIMT여“백맥산”ECG단독혹연합응용가현저개선모형대서신경공능평분、억제NVU세포조망、증가Bcl-2단백적표체，강저Caspase-3단백적표체。결론：CIMT급“백맥산”ECG가보호MCAO／R모형대서신경공능，기궤제가능여기항NVU세포조망，조절조망조공인자단백표체유관，차이자련용비이자단용구유경가적보호효과。
Objective： To study the neuroprotective effects and related mechanisms of constraint -induced movement therapy（ CI- MT）, effective compounds group （ECG） of ＂Baimai Powder＂ and combination of the two on cerebral ischemia- reperfusion inju- ry in rats. Method： 75 adult Sprague Dawley rats were randomly divided into 6 groups： sham- operated group （Sham）, middle cerebral artery occlusion/reperfusion group（ MCAO/R）, extract of Ginkgo Biloba group （EGB）, constraint - induced movement therapy group （CIMT） ,＂ Baimai Powder＂ ECG group （ECG）, constraint -induced movement therapy ＋ ＂Baimai Powder＂ ECG group（CIMT ＋ ECG）. The model of middle cerebral artery occlusion（MCAO） was established by thread ligation method, ische- mia 2h then reperfusion 24h, then the neurological score was taken , CIMT ＋ ECG group and ECG group were ig with the dosage of 155 mg/kg/d, EGB group was ig with the dosage of 5.6 mg/kg/d, the equivalent vehicle was given to the other groups, all groups were ig for 7d, respectively, Sports rehabilitation model was established to the CIMT group and CIMT ＋ ECG group at the same time. Then the protein expression of Bcl - 2 and Caspase - 3 of the cells of the neurovascular unit （NVU） were detected by immuno - hisochemistry, apoptosis cells of the NVU were detected by means of TdT - Mediated dUTP nick end labeling （TUNEL）. Result： CIMT and ECG of ＂Baimai Powder＂ singly or in combination can significantly improve the neurological score, restrain the apoptosis cells of the NVU, increase the expression of Bcl - 2 positive cells and decrease the expression of Caspase -3 positive cells. Conclusion： CIMT and ECG of ＂Baimai Powder＂ can remarkably improve the neural behavioral func- tions of model rats by resisting the apoptosis cells of the NVU and adjusting protein expression of apoptosis - regulating cyto- kines. The curative effect of the combination of CIMT and the ECG of ＂Baimai Powder＂ is much better.
