CAJ | 학술논문

纳米铂粒子( PtNPs)具有良好的生物相容性及高的催化性能,利用恒电位法将DNA生物分子电沉积在PtNPs修饰电极表面,得到一纳米结构的导电薄膜,极大地增大了电极的比表面积,结合Nafion的高选择性,制备了一种新型的Nafion/DNA/PtNPs复合膜修饰电极,研究了多巴胺( DA)在该修饰电极上的电化学行为,利用示差脉冲伏安法( DPV)对DA进行了定量分析.结果证明,该复合膜修饰电极大大提高了DA的电化学响应,在0.1 mol/L pH 7.0磷酸盐缓冲溶液( PBS)中,DA的示差脉冲伏安峰电流与其浓度在0.01~0.1μmol/L和0.1~6.0μmol/L两个范围内呈良好的线性关系,检出限可达3.3 nmol/L.此外,该修饰电极可以经受较高浓度抗坏血酸( AA)和尿酸( UA)的干扰,用于盐酸多巴胺注射液中DA含量的测定,结果满意.
납미박입자( PtNPs)구유량호적생물상용성급고적최화성능,이용항전위법장DNA생물분자전침적재PtNPs수식전겁표면,득도일납미결구적도전박막,겁대지증대료전겁적비표면적,결합Nafion적고선택성,제비료일충신형적Nafion/DNA/PtNPs복합막수식전겁,연구료다파알( DA)재해수식전겁상적전화학행위,이용시차맥충복안법( DPV)대DA진행료정량분석.결과증명,해복합막수식전겁대대제고료DA적전화학향응,재0.1 mol/L pH 7.0린산염완충용액( PBS)중,DA적시차맥충복안봉전류여기농도재0.01~0.1μmol/L화0.1~6.0μmol/L량개범위내정량호적선성관계,검출한가체3.3 nmol/L.차외,해수식전겁가이경수교고농도항배혈산( AA)화뇨산( UA)적간우,용우염산다파알주사액중DA함량적측정,결과만의.
A new nanocomposite film modified electrode was preparaed by electrodepositing DNA at the platinum nanoparticles(PtNPs)surface and then coating Nafion on the composite film. This modified electrode(Nafion/DNA/PtNPs) was applied to detect dopamine( DA) by using differential pulse voltammetry( DPV) . The result indicates that the Nafion/DNA/PtNPs nanocomposite film has electrocatalytic effect on the oxidation of DA and results in an obvious en-hancement of the current response. In 0. 1 mol/L pH 7. 0 phosphate buffer solution(PBS),the DPV peak heights are linear with DA concentration in the range of 0. 01-0. 1 μmol/L and 0. 1-6. 0 μmol/L with the detection limit of 3. 3 nmol/L(S/N=3). In addition,the interferences of uric acid(UA)and ascorbic acid(AA)can be effectively diminished. Moreover,the Nafion/DNA/PtNPs/GCE was applied to the DA detection in dopamine hydrochloride injection with satisfied results.