中国兽药杂志
中國獸藥雜誌
중국수약잡지
CHINESE JOURNAL OF VETERINARY DRUG
2012年
6期
19-22
,共4页
高效液相色谱-串联质谱法%同位素内标%莱克多巴胺%猪尿液%解离提取
高效液相色譜-串聯質譜法%同位素內標%萊剋多巴胺%豬尿液%解離提取
고효액상색보-천련질보법%동위소내표%래극다파알%저뇨액%해리제취
high performance liquid chromatography tandem mass spectrometry (HPLC - MS/MS)%isotope internal standard%ractopamine%pig urine%dissociation extraction
采用同位素内标法和高效液相-串联质谱法对猪尿莱克多巴胺阳性样品进行三种不同解离处理方法比较试验。结果表明，三种方法差异明显，β-葡萄糖醛苷酶／芳基硫酸酯酶在37℃酶解16h效果最好，在60℃酶解2h效果次之，0．1％高氯酸酸解提取效果最差。
採用同位素內標法和高效液相-串聯質譜法對豬尿萊剋多巴胺暘性樣品進行三種不同解離處理方法比較試驗。結果錶明，三種方法差異明顯，β-葡萄糖醛苷酶／芳基硫痠酯酶在37℃酶解16h效果最好，在60℃酶解2h效果次之，0．1％高氯痠痠解提取效果最差。
채용동위소내표법화고효액상-천련질보법대저뇨래극다파알양성양품진행삼충불동해리처리방법비교시험。결과표명，삼충방법차이명현，β-포도당철감매／방기류산지매재37℃매해16h효과최호，재60℃매해2h효과차지，0．1％고록산산해제취효과최차。
Comparison tests of three different dissociation treatment methods on porcine urinary ractopamine positive samples using isotope internal standard method and high performance liquid chromatographytandem mass spectrometry （HPLC - MS/MS ） were carried out. The results showed that three methods had obvious differences, the effect of β -glucuronidase/sulfatase enzyme for 16 hours at 37℃ was the best, enzymatic hydrolysis for 2 hours at 60℃ was the better, 0.1% perchloric acid solution for extraction effect was the worst.
