CAJ | 학술논문

[目的]研究酶降解法制备的壳寡糖对黄瓜黑星病病菌的抑制机理。【方法】测定壳寡糖对黄瓜黑星病菌菌丝发育、孢子萌发的抑制率，观察壳寡糖处理前后病菌菌丝形态及细胞膜透性变化。【结果】当壳寡糖处理浓度为0．2g／mL以上时，可完全抑制病菌菌丝生长；当处理浓度为O．1g／mL以上时，可完全抑制病菌孢子萌发。显微观察，浓度为O．8mg／m1．的壳寡糖处理可诱导菌丝分枝增多、菌体分隔增加，分生孢子形成受到抑制。此外壳寡糖能够引起菌丝细胞膜透性发生变化，造成菌丝体内含物的渗漏。【结论】该研究可为壳聚糖对黄瓜黑星病病菌的抑制提供参考依据。
[목적]연구매강해법제비적각과당대황과흑성병병균적억제궤리。【방법】측정각과당대황과흑성병균균사발육、포자맹발적억제솔，관찰각과당처리전후병균균사형태급세포막투성변화。【결과】당각과당처리농도위0．2g／mL이상시，가완전억제병균균사생장；당처리농도위O．1g／mL이상시，가완전억제병균포자맹발。현미관찰，농도위O．8mg／m1．적각과당처리가유도균사분지증다、균체분격증가，분생포자형성수도억제。차외각과당능구인기균사세포막투성발생변화，조성균사체내함물적삼루。【결론】해연구가위각취당대황과흑성병병균적억제제공삼고의거。
[Objective] The aim was to study the inhibition mechanism of the chitosan-oligosaccharides prepared through enzymatic hydrolysis to Cladosporium cueumerinum. [Method] The inhibition rate of the ehitosan-oligosaceharides upon the hyphae and the spore germination of Cladosporium cueumerinum were determined. The morphology shape of hyphae and the cell membrane permeability of the pathogen were observed before and after the treatment [Result] When the concentration of the chitosan-oligosaccharides was 0.2 g/mL, the hyphae growth were inhibited. When the concentration was 0. 1g/mL, the spore germination were inhibited. According to the microscope observation, the chitosan-oligosaccharides with concentration of 0.8 mg/mL induced the morphological change, including excessive branching and hyphal segments in the new hyphal of Cladosporium cueumerimum. The formation of conidiospore was inhibited. The chitosan-oligosaccharides could induced the permeability of the cell membrane and the inclusion leakage of mycelium. [Conelusion] This study could provide references for the inhibition mechanism of Cladosporium cueumerinum.