福建分析测试
福建分析測試
복건분석측시
FUJIAN ANALYSIS & TESTING
2012年
3期
1-5
,共5页
氨基黑10B%DNA%作用机理%沟槽结合
氨基黑10B%DNA%作用機理%溝槽結閤
안기흑10B%DNA%작용궤리%구조결합
Amide black 10B%DNA%action mechanism%groove binding
采用电子吸收光谱、粘度测定和电化学等方法研究了氨基黑10B（AB）与鲱鱼精DNA在B-R缓冲溶液中的相互作用。电子吸收光谱实验表明,DNA的加入能引起AB在618 nm处的最大吸收峰出现增色效应,说明二者的结合不属于典型的静电或嵌插结合模式,而可能通过沟槽方式结合,且伴随有氢键作用。粘度法实验证实了这一结果。电化学实验进一步表明DNA的加入能引起AB分子的扩散速率降低,从而导致AB的氧化峰电流出现减小。同时考察了AB与DNA结合前后的电子转移速率,结果表明AB与DNA之间形成了一种非电活性的超分子复合物。
採用電子吸收光譜、粘度測定和電化學等方法研究瞭氨基黑10B（AB）與鯡魚精DNA在B-R緩遲溶液中的相互作用。電子吸收光譜實驗錶明,DNA的加入能引起AB在618 nm處的最大吸收峰齣現增色效應,說明二者的結閤不屬于典型的靜電或嵌插結閤模式,而可能通過溝槽方式結閤,且伴隨有氫鍵作用。粘度法實驗證實瞭這一結果。電化學實驗進一步錶明DNA的加入能引起AB分子的擴散速率降低,從而導緻AB的氧化峰電流齣現減小。同時攷察瞭AB與DNA結閤前後的電子轉移速率,結果錶明AB與DNA之間形成瞭一種非電活性的超分子複閤物。
채용전자흡수광보、점도측정화전화학등방법연구료안기흑10B（AB）여비어정DNA재B-R완충용액중적상호작용。전자흡수광보실험표명,DNA적가입능인기AB재618 nm처적최대흡수봉출현증색효응,설명이자적결합불속우전형적정전혹감삽결합모식,이가능통과구조방식결합,차반수유경건작용。점도법실험증실료저일결과。전화학실험진일보표명DNA적가입능인기AB분자적확산속솔강저,종이도치AB적양화봉전류출현감소。동시고찰료AB여DNA결합전후적전자전이속솔,결과표명AB여DNA지간형성료일충비전활성적초분자복합물。
The interaction between amide black 10B （AB） and DNA in B-R buffer solution was investigated by the electronic absorption spectra, viscometry and electrochemistry. The characteristic absorption peak at 618 nm of AB showed obvious hyperchromic effect after interaction with DNA, suggesting that the interaction of AB with DNA was neither ascribed to intercalation nor eleotrostatic action, but via a groove binding mode with some hydrogen bonding, which was testified by viscosity measurement. Electrochemical experiments further suggested that the diffusion rate of AB was lowed after interaction with DNA, resulted in the decrease of oxidation peak currents. The results of electron-transferred rate suggested that an electro-inactive complex was formed between AB and DNA.
