CAJ | 학술논문

对同型巴蜗牛（Bradybaena similaris）蛋白腺提取液凝集活性进行测定，初步提取其凝集素并进行部分性质鉴定．结果表明：在同型巴蜗牛的蛋白腺中存在凝集素，具有血球凝集活性，能够凝集9种动物红细胞，其中，对家兔红细胞的凝集活性最高，可达2^8，不过，其对家兔红细胞的凝集活性明显依赖于Ca2＋；在pH值为7．0～8．0的范围内其凝集活性较稳定，保持100％的凝集活力；温度为40℃持续作用10min后就失去活性；向同型巴蜗牛蛋白腺提取液加人45％的固体硫酸铵使凝集素沉淀，再用不同饱和度的硫酸铵溶液依次对沉淀物进行抽提，抽提物的比活力提高了约2．56倍，总活力回收为20．00％．
대동형파와우（Bradybaena similaris）단백선제취액응집활성진행측정，초보제취기응집소병진행부분성질감정．결과표명：재동형파와우적단백선중존재응집소，구유혈구응집활성，능구응집9충동물홍세포，기중，대가토홍세포적응집활성최고，가체2^8，불과，기대가토홍세포적응집활성명현의뢰우Ca2＋；재pH치위7．0～8．0적범위내기응집활성교은정，보지100％적응집활력；온도위40℃지속작용10min후취실거활성；향동형파와우단백선제취액가인45％적고체류산안사응집소침정，재용불동포화도적류산안용액의차대침정물진행추제，추제물적비활력제고료약2．56배，총활력회수위20．00％．
The agglutination activity of albumen gland extract from a Bradybaena similaris was measured. The agglutinin was extracted and part of its properties was identified. The results showed agglutinin existed in the albumen gland of bradybaena similaris ; this agglutinin had blood-cell agglutination activity, and was able to agglutinate 9 kinds of animal red blood-cells, of which the domestic rabbit＇s was agglutinated with highest activity up to 2s. However, the agglutination activity on domestic rabbit＇s red blood - cell was significantly dependent on Ca2＋. It was stable in pH 7.0 -8.0 with 100 % activity, and was lost after continuous functio-ning for 10 minutes in 40 ℃ temperature, which indicated its heat resistance was poor. 45 % solid ammonium sulfate was added into the albumen gland extract from a bradybaena similaris to precipitate the agglutinin, and then the precipitates were extracted respectively by using ammonium sulfate solution with different saturation. The specific activity of the extract was. increased by about 2. 56 times with total activity recovery of 20. 00%.