CAJ | 학술논문

利用吸光值、琼脂糖电泳及PCR差异扩增等指标，对酚一氯仿法、水煮法、碱裂解法3种方法制备的鳗鲡病原菌DNA模板进行了差异分析．结果表明：1）不同方法提取的病原菌模板DNA的浓度、纯度及分子质量大小存在差异，但以这3种方法提取的病原菌DNA为模板，均能成功扩增到细菌16SrD．NA和外膜蛋白的保守序列．其中，酚一氯仿提取的模板DNA浓度较低、纯度较高；水煮法和碱裂解法提取的模板DNA浓度较高、纯度较低．2）电泳显示，3种方法获得的模板DNA扩增出的16SrDNA保守序列条带均一，没有显著差异，但在外膜蛋白保守序列的扩增中却因菌种和模板提取方法的不同而存在差异．
이용흡광치、경지당전영급PCR차이확증등지표，대분일록방법、수자법、감렬해법3충방법제비적만려병원균DNA모판진행료차이분석．결과표명：1）불동방법제취적병원균모판DNA적농도、순도급분자질량대소존재차이，단이저3충방법제취적병원균DNA위모판，균능성공확증도세균16SrD．NA화외막단백적보수서렬．기중，분일록방제취적모판DNA농도교저、순도교고；수자법화감렬해법제취적모판DNA농도교고、순도교저．2）전영현시，3충방법획득적모판DNA확증출적16SrDNA보수서렬조대균일，몰유현저차이，단재외막단백보수서렬적확증중각인균충화모판제취방법적불동이존재차이．
By the UV absorbance values, maps of agarose gel electrophoresis and the PCR amplification results, this study analyzed the differentiations of those templates DNA extracted from three pathogenic bacte- ria strains isolated from eels by three methods of water boiling, alkali lyase and phenol-chloroform. The re- sults indicated that the templates DNA presented some differences in extracted concentration, purity and mole- cules weights; and the DNA extracted by the phenol-chloroform method has lower concentration, higher puri- ty and smaller molecules weights comparing with those DNA templates extracted by the other two methods. And all the templates DNA showed no difference in amplifying the conserved sequences of 16S rDNA, while the showed some differences in amplifying the partial out membrane protein （OMP） gene from the three bac- teria strains.