CAJ | 학술논문

研究了绿液预处理玉米秸秆对里氏木霉产纤维素酶的影响。通过正交试验考察3个预处理条件对玉米秸秆产纤维素酶的影响,从极差和方差分析可知,对绿液预处理玉米秸秆产纤维素酶的影响程度由大到小依次是总碱量、蒸煮温度、硫化度,最大滤纸酶活（FPA）达到2.6 IU/mL。比较在蒸煮温度140℃和170℃,总碱量4%,硫化度0、20%、30%与40%下所产的FPA,经综合评定,在最优条件下蒸煮温度140℃,总碱量4%和硫化度0时可以尽量避免原料损失的前提下,保证FPA的大小,结果表明,里氏木霉利用绿液预处理玉米秸秆来产纤维素酶是可行的。以蒸煮温度140℃,总碱量4%和硫化度0的条件下绿液预处理得到的玉米秸秆为碳源,碳源浓度为12 g/L,FPA和β-葡萄糖苷酶活（β-GA）分别达到2.5 IU/mL和1.3 IU/mL,产酶周期5 d。
연구료록액예처리옥미갈간대리씨목매산섬유소매적영향。통과정교시험고찰3개예처리조건대옥미갈간산섬유소매적영향,종겁차화방차분석가지,대록액예처리옥미갈간산섬유소매적영향정도유대도소의차시총감량、증자온도、류화도,최대려지매활（FPA）체도2.6 IU/mL。비교재증자온도140℃화170℃,총감량4%,류화도0、20%、30%여40%하소산적FPA,경종합평정,재최우조건하증자온도140℃,총감량4%화류화도0시가이진량피면원료손실적전제하,보증FPA적대소,결과표명,리씨목매이용록액예처리옥미갈간래산섬유소매시가행적。이증자온도140℃,총감량4%화류화도0적조건하록액예처리득도적옥미갈간위탄원,탄원농도위12 g/L,FPA화β-포도당감매활（β-GA）분별체도2.5 IU/mL화1.3 IU/mL,산매주기5 d。
Abstract：The effect of green liquid pretreated corn stover on cellulase production of Trichoderma reesei was investigated. Inspecting the influence of pretreatment conditions on cellulase production of corn stover at different cooking temperature, total alkali charge and sulfidity by orthogonal experiment, we could conclude from range and variance analysis that the extent of impact to cellucase production of green liquor pretreated corn stover was in turn total alkali charge, cooking temperature and sultidity. The optimum filter paper activity （FPA） reached 2.6 IU/mL. FPA productions from corn stover oretreated bv cooking temperature140℃ and 170 ℃, total alkali charge 4 %, sulfidity 0, 20 %, 30 % and 40 %, were comprehensively compared and evaluated. It was found that the optimum conditions, i. e. , cooking temperature 140℃, total alkali charge 4 %, sulfidity 0, might ensure the value of FPA on and avoid the loss of materials. The results showed that green liquid pretreatment for corn stoverwas feasible for cellulase production of Trichoderma reesei. Taking corn stover pretreated by cooking temperature 140 ℃, total alkali charge 4% and sulfidity 0 as carbon sources whose concentration was 12 g/L, FPA and β-glucosidase activity（β-GA） were up to 2.5 IU/mL and 1.3 IU/mL respectively. The enzyme production period was 5 days.