酿酒
釀酒
양주
LIQUOR MAKING
2012年
3期
65-67
,共3页
任小虎%饶铖乐%史路路%陈茂彬
任小虎%饒鋮樂%史路路%陳茂彬
임소호%요성악%사로로%진무빈
人参皂甙%保健酒%HPLC%香草醛比色法
人參皂甙%保健酒%HPLC%香草醛比色法
인삼조대%보건주%HPLC%향초철비색법
Ginsenoside%Healthy wine%HPLC%Vanillin colorimetry
应用高效液相色谱法和香草醛比色法分别测定保健酒中人参皂甙的含量。高效液相色谱法采用线性梯度洗脱同时测定6种主要人参皂甙Rg1、Re、Rb1、Rc、Rb2、Rd。比色法以香草醛-高氯酸为显色剂,人参皂甙Re为对照品,测定人参总皂甙的含量。两种方法均简便、准确、灵敏度高、重复性好,适用于保健酒中人参皂甙的测定。
應用高效液相色譜法和香草醛比色法分彆測定保健酒中人參皂甙的含量。高效液相色譜法採用線性梯度洗脫同時測定6種主要人參皂甙Rg1、Re、Rb1、Rc、Rb2、Rd。比色法以香草醛-高氯痠為顯色劑,人參皂甙Re為對照品,測定人參總皂甙的含量。兩種方法均簡便、準確、靈敏度高、重複性好,適用于保健酒中人參皂甙的測定。
응용고효액상색보법화향초철비색법분별측정보건주중인삼조대적함량。고효액상색보법채용선성제도세탈동시측정6충주요인삼조대Rg1、Re、Rb1、Rc、Rb2、Rd。비색법이향초철-고록산위현색제,인삼조대Re위대조품,측정인삼총조대적함량。량충방법균간편、준학、령민도고、중복성호,괄용우보건주중인삼조대적측정。
To compare HPLC with vanillin colorimetry in determination of ginsenoside in healthy wine. The ginsenosides of Rgl, Re, Rbl, Rc, Rb2 and Rd were detemained by HPLC Linear gradient elution. Meanwhile, total contents of various ginsenosides were quantified by vanillin colorimetry with ginsenoside Re as reference.The two methods were simple, accurate, sensitive and reproducible and can be used for determination ginsenoside in healthy wine.