深圳职业技术学院学报
深圳職業技術學院學報
심수직업기술학원학보
JOURNAL OF SHENZHEN POLYTECNIC
2012年
3期
64-66
,共3页
杨懋勋%单振菊%陈志云%郑成业
楊懋勛%單振菊%陳誌雲%鄭成業
양무훈%단진국%진지운%정성업
莫娜百合%组织培养%快速繁殖%条件优化
莫娜百閤%組織培養%快速繁殖%條件優化
막나백합%조직배양%쾌속번식%조건우화
Lilium Mona%tissue culture%rapid propagation%conditional optimization
对观赏百合品种莫娜鳞片进行组培快繁条件的优化研究.结果表明,使用75%酒精消毒10s,0.1%升汞处理10min,最后用无菌水清洗5次灭菌效果最好,污染率最低;MS+0.5mg/L6-BA+1mg/LNAA为最佳诱导培养基.
對觀賞百閤品種莫娜鱗片進行組培快繁條件的優化研究.結果錶明,使用75%酒精消毒10s,0.1%升汞處理10min,最後用無菌水清洗5次滅菌效果最好,汙染率最低;MS+0.5mg/L6-BA+1mg/LNAA為最佳誘導培養基.
대관상백합품충막나린편진행조배쾌번조건적우화연구.결과표명,사용75%주정소독10s,0.1%승홍처리10min,최후용무균수청세5차멸균효과최호,오염솔최저;MS+0.5mg/L6-BA+1mg/LNAA위최가유도배양기.
Optimization of sterilization and induction conditional for tissue culture and rapid propagation of Lilium Mona were studied. The results show that when sterilized for 10s by 75% alcohol, treated for 10min by 0.1% mercuric chloride, and then cleaned 5 times by sterile water, the lowest contamination rate can be achieved. It is also pointed out that the best induction medium is MS+0.5 mg/L 6-BA+ 1 mg/L NAA.