CAJ | 학술논문

采用正交试验优化双孢蘑菇的液体培养基配方，发现黄豆粉、葡萄糖、硫酸镁、以及玉米粉和葡萄糖的交互作用对双孢蘑菇的液体培养有显著影响，磷酸二氢钾、麦辣汁对实验结果无显著影响。双孢蘑菇最佳液体培养基配方为：黄豆粉2％，玉米粉2％，葡萄糖2％，磷酸二氢钾0．05％，硫酸镁0．20％，麦麸0．20％。把双孢蘑菇液体菌种接入小麦栽培种中，茵丝表现出生长快的优势，生长速度较为一致，满瓶时间短，证明利用液体菌种制备栽培种比固体菌种具有更大的优势。
채용정교시험우화쌍포마고적액체배양기배방，발현황두분、포도당、류산미、이급옥미분화포도당적교호작용대쌍포마고적액체배양유현저영향，린산이경갑、맥랄즙대실험결과무현저영향。쌍포마고최가액체배양기배방위：황두분2％，옥미분2％，포도당2％，린산이경갑0．05％，류산미0．20％，맥부0．20％。파쌍포마고액체균충접입소맥재배충중，인사표현출생장쾌적우세，생장속도교위일치，만병시간단，증명이용액체균충제비재배충비고체균충구유경대적우세。
According to the orthogonal experimental design method, the interaction was found between the maize meal and the glucose for Agaricus bisporus in liquid culture. The experiment has an optimum result when their ratio is three to two. The optimum prescription of the culture media could be ascertained as follows： soybean flour 2%, maize meal 2%, glucose 2%, KH：PO4 0.05% , MgS04 0.20% , and wheat bran 0.05%. The mushroom spawns of Agaricus bisporus grown in wheat kernels medium was displayed better characters inoculated with the liquid culture seed than the wheat kernels culture seed on superiority of quick growth, consistent growth speed of hypha and the full bottle time. It was concluded that this liquid culture may be used as suitable original seed for the mushroom spawns seed of Agaricus bisporus