酿酒科技
釀酒科技
양주과기
LIQUOR-MAKING SCIENCE & TECHNOLOGY
2012年
2期
83-86
,共4页
冯东%王丙莲%梁晓辉%李雪梅%李大海
馮東%王丙蓮%樑曉輝%李雪梅%李大海
풍동%왕병련%량효휘%리설매%리대해
生物传感器%酒%乙醇%测定分析
生物傳感器%酒%乙醇%測定分析
생물전감기%주%을순%측정분석
biosensor%liquor%ethanol%determination
利用酶固定化技术,制备乙醇生物传感器,并用其测定酒中乙醇含量。结果表明,生物传感器法测定酒中乙醇含量快速而准确,检测时间为20 s,回收率为99.3%～101.2%,相对标准偏差为0.97%～1.50%;检测灵敏度高,检出限为1 mg/100 mL;与气相色谱法、密度瓶法相比较,差异不显著（P〉0.05）;且具有操作简单、成本低廉、专一性强等优势,有望成为酒类乙醇含量测定的新方法、新手段。
利用酶固定化技術,製備乙醇生物傳感器,併用其測定酒中乙醇含量。結果錶明,生物傳感器法測定酒中乙醇含量快速而準確,檢測時間為20 s,迴收率為99.3%～101.2%,相對標準偏差為0.97%～1.50%;檢測靈敏度高,檢齣限為1 mg/100 mL;與氣相色譜法、密度瓶法相比較,差異不顯著（P〉0.05）;且具有操作簡單、成本低廉、專一性彊等優勢,有望成為酒類乙醇含量測定的新方法、新手段。
이용매고정화기술,제비을순생물전감기,병용기측정주중을순함량。결과표명,생물전감기법측정주중을순함량쾌속이준학,검측시간위20 s,회수솔위99.3%～101.2%,상대표준편차위0.97%～1.50%;검측령민도고,검출한위1 mg/100 mL;여기상색보법、밀도병법상비교,차이불현저（P〉0.05）;차구유조작간단、성본저렴、전일성강등우세,유망성위주류을순함량측정적신방법、신수단。
Ethanol biosensor based on enzyme immobilization technology was used to determinate ethanol content in liquor.The results showed that the biosensor could measure ethanol content rapidly and accurately,with response time of 20 s,recovery ratio of 99.3 %～101.2 %,and repeatability relative standard deviation（RSD） 0.97 %～1.50 %.There was no significant difference（P〉0.05） in determination results between biosensor,gas chromatography（GC） and density bottle method.Besides,it had the advantages such as high sensitivity（detection limit was 1 mg/100 mL）,easy operation,low cost and high specificity.It seemed that biosensor would become the novel method of ethanol detection in liquor.
