徐州工程学院学报：自然科学版
徐州工程學院學報：自然科學版
서주공정학원학보：자연과학판
Journal of Xuzhou Istitute of Technology
2012年
2期
16-20
,共5页
山苍子%CO2超临界萃取%抗氧化活性测定%夹带剂
山蒼子%CO2超臨界萃取%抗氧化活性測定%夾帶劑
산창자%CO2초림계췌취%항양화활성측정%협대제
Litsea cubeba%supercritical carbon dioxide extraction%antioxidant activity test%co-solvent
应用二氧化碳超临界萃取法提取山苍子油，以正交设计法考察萃取温度、压力、流量、时间对提取效率的影响，与此同时，以DPPH法测定山苍子油的抗氧化活性，并首次研究了夹带剂使用与不同提取时段对山苍子油提取率及其抗氧化效能的影响．结果表明：在50℃、25MPa压力和40L／h流量时，油得率可高达36．7％；单位时间内山苍子油得率随着时间推延呈锐减趋势，第1时段（0～20min）占总出油量的80．86％～97．11％；但从抗氧化效能看，第2时段（20～40min）得到的山苍子油透明度高，抗氧化效果也明显优于前者；在超临界萃取过程中加入夹带剂可以显著提高山苍子油得率及山苍子油的抗氧化活性，以乙酸乙酯为夹带剂，萃取率最高可达45．88％；以甲醇为夹带剂时，山苍子油的抗氧化活性达到最佳值，DPPH法测得IC50仅为0．30g／L，而对照组IC50。为7．63g／L．
應用二氧化碳超臨界萃取法提取山蒼子油，以正交設計法攷察萃取溫度、壓力、流量、時間對提取效率的影響，與此同時，以DPPH法測定山蒼子油的抗氧化活性，併首次研究瞭夾帶劑使用與不同提取時段對山蒼子油提取率及其抗氧化效能的影響．結果錶明：在50℃、25MPa壓力和40L／h流量時，油得率可高達36．7％；單位時間內山蒼子油得率隨著時間推延呈銳減趨勢，第1時段（0～20min）佔總齣油量的80．86％～97．11％；但從抗氧化效能看，第2時段（20～40min）得到的山蒼子油透明度高，抗氧化效果也明顯優于前者；在超臨界萃取過程中加入夾帶劑可以顯著提高山蒼子油得率及山蒼子油的抗氧化活性，以乙痠乙酯為夾帶劑，萃取率最高可達45．88％；以甲醇為夾帶劑時，山蒼子油的抗氧化活性達到最佳值，DPPH法測得IC50僅為0．30g／L，而對照組IC50。為7．63g／L．
응용이양화탄초림계췌취법제취산창자유，이정교설계법고찰췌취온도、압력、류량、시간대제취효솔적영향，여차동시，이DPPH법측정산창자유적항양화활성，병수차연구료협대제사용여불동제취시단대산창자유제취솔급기항양화효능적영향．결과표명：재50℃、25MPa압력화40L／h류량시，유득솔가고체36．7％；단위시간내산창자유득솔수착시간추연정예감추세，제1시단（0～20min）점총출유량적80．86％～97．11％；단종항양화효능간，제2시단（20～40min）득도적산창자유투명도고，항양화효과야명현우우전자；재초림계췌취과정중가입협대제가이현저제고산창자유득솔급산창자유적항양화활성，이을산을지위협대제，췌취솔최고가체45．88％；이갑순위협대제시，산창자유적항양화활성체도최가치，DPPH법측득IC50부위0．30g／L，이대조조IC50。위7．63g／L．
Extraction of volatile oils from the dry fruits of Litsea cubeba with supercritical carbon dioxide was studied. The influence of temperature, pressure, CO2 flow rate and time on the efficiency of extraction was examined by orthogonal experiment. Meantime, the antioxidant activity of Litsea cubeba oil was measured by the DPPH test, and it was studied for the first time bow the application of co-solvent and the extraction time can affect the rate of extraction and the oil＇s antioxidant activity. The results show that the optimal extracting conditions are determined as pressure 25 MPa, temperature 50℃, CO2 flow rate 40 L/h, with largest extraction rate of volatile oils as 36.7 %. The extraction time can make big difference on that from the first 20 min. It is proved that adding the co-solvent can improve extract＇s total yield and antioxidant activity. The extraction rates can reach 45.88 % when acetic ether used as co-solvent. The best antioxidant activity can be achieved when methyl alcohol is used as co-solvent, with the IC50 measured by DPPH method as low as 0.30 g/L, compared with 7.63 g/L of CK.
