CAJ | 학술논문

运用PCR技术,扩增了梭鱼和鲻鱼线粒体16S rRNA和COⅠ基因片段,并比较分析了其种间的序列差异。获得16S rRNA基因的540～560 bp碱基序列,出现26个碱基的插入与缺失位点;获得COⅠ基因的602～604 bp碱基序列,出现2个插入缺失位点;16S rRNA和COⅠ基因的序列中G平均含量最低,且（A＋T）含量高于（G＋C）含量,与其他鱼类中的16S rRNA和COⅠ基因片段研究结果相一致。在16S rRNA基因片段中,梭鱼出现1种单倍型,鲻鱼为2种单倍型;在COⅠ基因片段中,梭鱼样品中检测到3个单倍型,鲻鱼样品中检测到5个单倍型。以Takifugu poecilonotus为外群,构建的NJ系统发育树,基于16S rRNA和COⅠ基因序列获得的NJ系统树基本一致,鲻鱼和梭鱼种内个体分别聚为一支,显示16S rRNA和COⅠ基因适合于梭鱼和鲻鱼的物种鉴定。
운용PCR기술,확증료사어화치어선립체16S rRNA화COⅠ기인편단,병비교분석료기충간적서렬차이。획득16S rRNA기인적540～560 bp감기서렬,출현26개감기적삽입여결실위점;획득COⅠ기인적602～604 bp감기서렬,출현2개삽입결실위점;16S rRNA화COⅠ기인적서렬중G평균함량최저,차（A＋T）함량고우（G＋C）함량,여기타어류중적16S rRNA화COⅠ기인편단연구결과상일치。재16S rRNA기인편단중,사어출현1충단배형,치어위2충단배형;재COⅠ기인편단중,사어양품중검측도3개단배형,치어양품중검측도5개단배형。이Takifugu poecilonotus위외군,구건적NJ계통발육수,기우16S rRNA화COⅠ기인서렬획득적NJ계통수기본일치,치어화사어충내개체분별취위일지,현시16S rRNA화COⅠ기인괄합우사어화치어적물충감정。
The Ⅰ6S rRNA and cytochrome oxidase subunit I （COⅠ） gene fragments of Liza haematocheila and Mugil cephalus were amplified by PCR, and the sequence variation among interspecies were analyzed. A total of 26 insertion or deletion of sites were detected for 16S rRNA; and 2 insertion or deletion of sites were detected for（CO Ⅰ ） gene. The average content of G was the lowest, and the content of （A＋T） was higher than that of （G＋C）, which was similar to the results reported in other fish species. For 16S rRNA, one haplotype was observed in L. haematocheila and 2 haplotypes were observed in M. cephalus. As for CO Ⅰ gene fragments, 3 haplotypes were detected in L. haematocheila and 5 haplotypes were detected in M. cephalus. Takifugu poe-ble to molecular identification.