CAJ | 학술논문

以蚕豆叶片下表皮为材料，借助表皮条分析、免疫细胞化学染色、细胞核染色和激光扫描共聚焦显微镜技术，对一氧化氮（nitricoxide，NO）在光暗调控的蚕豆气孔运动中的作用进行探索。实验证明，NO专一性清除剂2，4-羧基苯-4，4，5，5-四甲基咪唑-l-氧-3-氧化物（cPrIO）和一氧化氮合酶（Nos）抑制剂NG-氮-L-精氨酸-甲酯（L-NAME）能够逆转黑暗诱导气孔关闭的效应，并能阻断黑暗诱导的蚕豆保卫细胞NO水平的提高。但二者对光下气孔孔径几乎没有影响。结果表明，光暗调控的蚕豆气孔运动与保卫细胞胞质内的NO水平有关，在保卫细胞细胞核中存在诱导型一氧化氮合酶（inducible-nitric喇desynthase，iNOS），而光暗条件下iNOS活性不同，进而引起NO水平变化并最终影响气孔开关。
이잠두협편하표피위재료，차조표피조분석、면역세포화학염색、세포핵염색화격광소묘공취초현미경기술，대일양화담（nitricoxide，NO）재광암조공적잠두기공운동중적작용진행탐색。실험증명，NO전일성청제제2，4-최기분-4，4，5，5-사갑기미서-l-양-3-양화물（cPrIO）화일양화담합매（Nos）억제제NG-담-L-정안산-갑지（L-NAME）능구역전흑암유도기공관폐적효응，병능조단흑암유도적잠두보위세포NO수평적제고。단이자대광하기공공경궤호몰유영향。결과표명，광암조공적잠두기공운동여보위세포포질내적NO수평유관，재보위세포세포핵중존재유도형일양화담합매（inducible-nitric나desynthase，iNOS），이광암조건하iNOS활성불동，진이인기NO수평변화병최종영향기공개관。
In this study, the abaxial epidermis of Vicia faba is used as materials, and role of NO in light/dark - regulated / stomatal movement is investigated by epidermal strip bioassay, immunocytochemical methods, carbolfuchsin staining and la- serseanning confocal microscopy （LSCM）. Our data shows that the darkness - induced stomata closure and NO generation in guard cell can be prevented by 2- （4 - carboxyphenyl） - 4,4,5,5 - tetmmethylimidazohne - 1 - oxyl - 3 - oxide （cP- TIO） and NG - nitro - L - Arg - methyl ester （L - NAME）, the specific NO scavenger and an inhibitor of NO synthase （NOS） respectively, which has no significant effect on stomatal aperture in light. So we suggest that light/darkness- regu- lated stomata] movement is related to the levels of NO in guard cell. In addition, we also indicate that in nucleus of V. faba guard cells plant possess inducible- nitric oxide synthase （iNOS）, and light/darkness - regulated NO levels in guard cells of Vicia faba is likely related to the change of iNOS activeness, and then results in the opening or closing of the stomata.