CAJ | 학술논문

目的运用变性高效液相色谱(denaturing high-performance liquid chromatography,DHPLC)技术检测肺炎支原体(mycoplasma pneumoniae,MP)基因型,了解儿童MP流行状况.方法对我院300例患儿鼻咽吸出物进行表型确证试验,采用PCR对标准菌株和MP表型阳性的临床菌株进行PCR扩增,扩增产物经DHPLC分析,通过与标准菌株色谱峰比对进行临床菌株基因分型.结果 300例患儿咽拭子经过24h培养后呈阳性110例.用特异引物扩增MP-129、MP-FH标准株和标本,分别得到2 280 bp和2580bp基因片段.110株临床菌株经DHPLC分析检测出107株是P1-Ⅰ型,均是1b亚型,3株是P1-Ⅱ型,均是2a亚型.结论运用DHPLC技术检测出本院儿童发生MP感染以P1-Ⅰ、1b亚型为主.
목적 운용변성고효액상색보(denaturing high-performance liquid chromatography,DHPLC)기술검측폐염지원체(mycoplasma pneumoniae,MP)기인형,료해인동MP류행상황.방법 대아원300례환인비인흡출물진행표형학증시험,채용PCR대표준균주화MP표형양성적림상균주진행PCR확증,확증산물경DHPLC분석,통과여표준균주색보봉비대진행림상균주기인분형.결과 300례환인인식자경과24h배양후정양성110례.용특이인물확증MP-129、MP-FH표준주화표본,분별득도2 280 bp화2580bp기인편단.110주림상균주경DHPLC분석검측출107주시P1-Ⅰ형,균시1b아형,3주시P1-Ⅱ형,균시2a아형.결론 운용DHPLC기술검측출본원인동발생MP감염이P1-Ⅰ、1b아형위주.
Objective To investigate the genetype distribution of mycoplasma pneumoniae(MP) by denaturing high-performance liquid chromatography(DHPLC).Methods A total of 300 cases nasopharyngeal aspirate were collected from our hospital.The MP genes of standard strains and clinical specimens isolates were amplified by PCR followed by DHPLC and genetype determination.Results A total of 110 cases were positive after 24 hours fermentation from 300 cases with pharyngeal swab.By the specific primers of MP-129,MP-FH standard strain and specimens,2 280 bp and 2 580 bp gene fragments were made out respectively.One hundred and ten strains of clinical isolates were detected by DHPLC.One hundred and seven strains of P1-Ⅰ were 1b subtype,3 were type P1-Ⅱ which were all 2a subtype.Conclusion The genetype of MP infection in children from our hospital is P1-Ⅰ,1b subtype by using DHPLC technology.