CAJ | 학술논문

本研究根据GenBank中鸭新城疫病毒（NDV）的F基因和鸭圆环病毒（DuCV）的V1／rep基因的保守序列，各设计一对特异性引物，并对二重PCR的扩增条件进行优化，建立了鸭NDV和DuCV的二重PCR检测方法。对混合样品进行扩增，得到2条大小为493bp（鸭NDV）和218bp（DuCV）的特异性条带，与预扩增片段相符。而对番鸭细小病毒、鸭瘟病毒、鸭肝炎病毒、鸭源小鹅瘟病毒、鸭H9亚型流感病毒、鸭疫里氏杆菌、大肠杆菌、禽多杀性巴氏杆菌等病原检测，结果为阴性。该方法的敏感性试验表明，鸭NDV的核酸最小量为40fg，DuCV为20龟。
본연구근거GenBank중압신성역병독（NDV）적F기인화압원배병독（DuCV）적V1／rep기인적보수서렬，각설계일대특이성인물，병대이중PCR적확증조건진행우화，건립료압NDV화DuCV적이중PCR검측방법。대혼합양품진행확증，득도2조대소위493bp（압NDV）화218bp（DuCV）적특이성조대，여예확증편단상부。이대번압세소병독、압온병독、압간염병독、압원소아온병독、압H9아형류감병독、압역리씨간균、대장간균、금다살성파씨간균등병원검측，결과위음성。해방법적민감성시험표명，압NDV적핵산최소량위40fg，DuCV위20구。
According to the sequences of duck NDV F gene and DuCV V1/rep gene in GenBank, two pairs of specific primers were designed, and the reaction conditions were optimized, and then a duplex PCR assay was developed for detection of Newcastle disease virus and circovirus in ducks. All samples containing Newcastle disease virus and circovirus could be amplified into two specific bands, 493 bp for duck Newcastle disease virus and 218 bp for duck circovirus by this duplex PCR, but no specific bands of the same sizes were amplified from other duck pathogens, such as Muscovy duck parvovirus, duck plague virus, duck hepatitis virus, gosling plague virus, duck H9 subtype avian influenza virus, Riemerella anatipestifer, E.coli, avian Pasteurella multocida. As little as 40 fg of duck NDV and 20 fg ofDuCV DNA could be detected.