CAJ | 학술논문

利用13条ISSR引物分析了70份四川地方性晾晒烟材料的遗传多样性．共扩增出107条带，平均每个引物扩增8．23条，引物的平均多态性比率为90．37％，聚类分析表明，品种间遗传相似指数的范围在0．46—0．87，平均遗传相似性系数为0．646．这表明其遗传差异不大．其中遗传相似性最大的品种为达州的土烟与新都的黑牛皮，遗传相似指数为0．46；遗传相似性最小的品种为什邡的红毛柳和巴毛柳，遗传相似指数为0．87．聚类和主成分分析一致表明，70份材料在遗传相似系数为0．646处分为5类，并且四川晒烟种质资源遗传多样性与其地理来源相关．ISSR标记能高效揭示亲缘关系很近的晾晒烟种质资源的亲缘关系，因此适用于烟草品种鉴定和遗传多样性研究．
이용13조ISSR인물분석료70빈사천지방성량쇄연재료적유전다양성．공확증출107조대，평균매개인물확증8．23조，인물적평균다태성비솔위90．37％，취류분석표명，품충간유전상사지수적범위재0．46—0．87，평균유전상사성계수위0．646．저표명기유전차이불대．기중유전상사성최대적품충위체주적토연여신도적흑우피，유전상사지수위0．46；유전상사성최소적품충위십방적홍모류화파모류，유전상사지수위0．87．취류화주성분분석일치표명，70빈재료재유전상사계수위0．646처분위5류，병차사천쇄연충질자원유전다양성여기지리래원상관．ISSR표기능고효게시친연관계흔근적량쇄연충질자원적친연관계，인차괄용우연초품충감정화유전다양성연구．
Genetic diversity of 70 Sichuan sun-cured tobacco cuhivars was analyzed by inter simple sequence repeats （ISSR）. 107 bands were identified using agarose gel eleetrophoresis. The average number of amplified DNA bands was 8.23 per primer, and the rate of polymorphic bands was 90.37%. Genetic similarity ranged from 0.46 to 0.87 through cluster analysis with UPGMA, which suggested the genetic diversity among these germplasms not obvious, and the average genetic similarity index was 0.646. Genetic similarity index between Hong maoliu and Ba maoliu was 0.87, indicating that their genetic diversity was similar, but genetic similarity index between Tu yan from Da zhou and Hei niupi from Xin du was 0.46, showing more difference of their genetic diversity. Cluster analysis and principal component analysis （PCA） were carried out. The results showed that the 70 Sichuan sun-cured tobacco cultivars were divided into five groups, deeply associated with their geographical distribution. ISSR analysis was suitable for variety identification and the research on genetic diversity of tobacco germplasm.