CAJ | 학술논문

目的：探索用胶体金免疫层析法快速检测胆酸钠含量。方法：用碳二亚胺法合成的免疫抗原胆酸钠-BSA,免疫新西兰白兔,制备抗胆酸钠多克隆抗体。用酶联免疫吸附法（ELISA）鉴定特异性抗体效价。制备胆酸钠—OVA抗原—胶体金复合物,组装检测试纸。结果：胆酸钠抗体的效价为1∶80 000,建立的胶体金快速测定法对胆酸钠的检测限为12μg/mL。结论：试纸条法是快速检测胆汁酸含量的有效方式,在临床检测方面具有广阔的运用前景。
목적：탐색용효체금면역층석법쾌속검측담산납함량。방법：용탄이아알법합성적면역항원담산납-BSA,면역신서란백토,제비항담산납다극륭항체。용매련면역흡부법（ELISA）감정특이성항체효개。제비담산납—OVA항원—효체금복합물,조장검측시지。결과：담산납항체적효개위1∶80 000,건립적효체금쾌속측정법대담산납적검측한위12μg/mL。결론：시지조법시쾌속검측담즙산함량적유효방식,재림상검측방면구유엄활적운용전경。
Objective：To develop colloidal gold immunochromatographic（IC） method for rapidly detecting the concentration of Sodium Cholate.Methods： Sodium Cholate-BSA immunogen was obtained via carbodiimide method.Then the immunoantigen was injected into New Zealand rabbits to prepare the anti-sodium cholate polyclona antibody according to routine immunity procedure.The specific antibody of Bile acid natrium was identified by indirect enzyme linked immunosorbent assay（ELISA）.Results： The sodium cholate-OVA antigen-colloidal gold compounds were made for test strip preparation.It was proved that the specificity of the antibody was 1 ∶ 80 000,and the detectability was 12 μg/mL by the method.Conclusion： Accordingly,IC test strip method is effective and sensitive for rapidly detecting sodium cholate,which will probably have a broad clinical application prospect.