CAJ | 학술논문

为观察灭活的双歧杆菌对小鼠肠液中SIgA水平的影响，将小鼠腹腔注射环磷酰胺造成免疫抑制动物模型，分别以新鲜BS肉汤培养基、耗尽培养上清（SCS）以及死、活双歧杆菌菌液进行灌胃。采用双抗体夹心法合并间接法检测肠液中SIgA的含量。结果显示灭活的双歧杆菌与双歧杆菌活菌均可提高免疫抑制小鼠肠液中SIgA的含量，二者的作用效果差异无显著性（P〉0．05），SCS也具有一定的免疫促进作用，但与双歧杆菌活菌相比差异有显著性（P〈0．05）。灭活的双歧杆菌具有与双歧杆菌活菌相同或相近的免疫学活性，
위관찰멸활적쌍기간균대소서장액중SIgA수평적영향，장소서복강주사배린선알조성면역억제동물모형，분별이신선BS육탕배양기、모진배양상청（SCS）이급사、활쌍기간균균액진행관위。채용쌍항체협심법합병간접법검측장액중SIgA적함량。결과현시멸활적쌍기간균여쌍기간균활균균가제고면역억제소서장액중SIgA적함량，이자적작용효과차이무현저성（P〉0．05），SCS야구유일정적면역촉진작용，단여쌍기간균활균상비차이유현저성（P〈0．05）。멸활적쌍기간균구유여쌍기간균활균상동혹상근적면역학활성，
To explore the effect of heat-killed bifidobacteria on the production of SIgA in intestinal juice of mice. Got immunosuppressing mice by intraperitoneally in jecting CTX. Five groups of mice were administrated orally with fresh BS culture medium, SCS, heat-killed bifidobacteria and live bifidobacteria. Evaluated the production SIgA of by double antibody sandwich methods and indirect of ELISA assay. Both heat-killed bifidobacteria and live bif ido bacteria could increase the production of SIgA, but there was no significant difference between two groups （P〉0.05）. SCS could improve the immunity of immunosupprossing mice in some degree, but it was a little weaker （P〈0.05）. Heat-killed bifidobacteria and live bifidobacteria had the same or similar biological activity and both could improve the production of SIgA in intestinal juice of mice.