CAJ | 학술논문

以橄榄油为碳源，采用富集和复筛的方法，于夏季从海口市食堂和汽修场等富油土壤中筛选出9株高产脂肪酶活性菌株，对其形态、生长和16SrRNA序列进行分析，结果表明：各菌株菌落皆呈暗绿色，镜检为革兰氏阴性、杆状单细胞，最适生长pH值为7，最适生长温度高于42℃，9株菌株的16SrDNA序列完全一致，皆为绿脓假单胞菌。
이감람유위탄원，채용부집화복사적방법，우하계종해구시식당화기수장등부유토양중사선출9주고산지방매활성균주，대기형태、생장화16SrRNA서렬진행분석，결과표명：각균주균락개정암록색，경검위혁란씨음성、간상단세포，최괄생장pH치위7，최괄생장온도고우42℃，9주균주적16SrDNA서렬완전일치，개위록농가단포균。
Nine high producing lipase strains were isolated from rich oil soil at canteens, sea sludge and garages from Haikou in hot summer. All these strains were Gram-negative and rod-shaped single cells and investigated in terms of morphology, growth and 16S rRNA gene sequences. All colonies of these strains were blue green on the plate under light microscope, indicating that the strains might secrete pigments of such color. The optimal growth conditions for these strains were pHT. 0 at the temperature of above 42℃. All the strains had completely the same 16S rRNA gene sequences as Pseudomonas aeruginosa, and hence are identified as P. aeruginosa.