CAJ | 학술논문

根据日本晴cab4基因序列（GenBank：AK104499.1）设计引物,用RT-PCR的方法从籼稻9311中克隆了叶绿素a/b结合蛋白基因的全长cDNA,命名为cab-9311（cab gene from 9311）。insilico分析表明：cab-9311与cab4基因同源性为99%,编码的蛋白含有244个氨基酸,与cab4基因编码的蛋白同源性为98%。蛋白分子质量为26.9kD,理论等电点为6.52。第54位～第216位氨基酸是一个典型的叶绿素a/b结合蛋白功能域（chlorophyll a/bbinding domain）。跨膜分析和蛋白质三级预测显示,该蛋白在C端有一个典型的跨膜区。亚细胞定位分析表明该蛋白定位于叶绿体,是一个叶绿体内囊体膜上的锚定蛋白。
근거일본청cab4기인서렬（GenBank：AK104499.1）설계인물,용RT-PCR적방법종선도9311중극륭료협록소a/b결합단백기인적전장cDNA,명명위cab-9311（cab gene from 9311）。insilico분석표명：cab-9311여cab4기인동원성위99%,편마적단백함유244개안기산,여cab4기인편마적단백동원성위98%。단백분자질량위26.9kD,이론등전점위6.52。제54위～제216위안기산시일개전형적협록소a/b결합단백공능역（chlorophyll a/bbinding domain）。과막분석화단백질삼급예측현시,해단백재C단유일개전형적과막구。아세포정위분석표명해단백정위우협록체,시일개협록체내낭체막상적묘정단백。
With the primer designed according to the Nipponbare cab4 gene sequence （GenBank： AK104499.1）, a full-length cDNA gene encoding the light-harvesting chlorophyll a/b-binding protein in rice 9311 was cloned by RT-PCR, and it was named cab-9311 （cab gene from 9311）. In silico analysis shows that the cab-9311 cDNA sequence has 99% homology to the cab4 cDNA sequence. Cab-9311 encodes a protein of 244 amino acids which has 98% homology to the protein encoded by cab4 gene. The molecular weight of that protein is about 26.9 kD and its isoelectric point is 6.52. The amino acid residues 54-216 of that protein is a chlorophyll a/b binding domain. The analysis of transmembrane protein and the prediction of protein tertiary structure show a typical transmembrane domain is located in the C-terminus of that protein. The prediction of the subcellular location shows that protein is located in the chloroplast. All the results give a clue that the protein encoded by cab-9311 is embedded in the thylakoid membranes.