CAJ | 학술논문

采用ISSR分子标记技术,分析78份杧果种质的遗传多样性。从100条引物中筛选出12条优选引物,共扩增出148条DNA条带,其中多态性带142条,多态性百分率为95.95%。聚类分析结果将78份杧果种质分为5大类。在5大类资源中,大部分种质（87.34%）都聚在第Ⅰ类,表明大部分杧果种质之间亲缘关系较近,供试种质之间能互相区分开来并表现出丰富的遗传多样性。
채용ISSR분자표기기술,분석78빈망과충질적유전다양성。종100조인물중사선출12조우선인물,공확증출148조DNA조대,기중다태성대142조,다태성백분솔위95.95%。취류분석결과장78빈망과충질분위5대류。재5대류자원중,대부분충질（87.34%）도취재제Ⅰ류,표명대부분망과충질지간친연관계교근,공시충질지간능호상구분개래병표현출봉부적유전다양성。
ISSR marker was used to analyze genetic diversities among 78 mango germplasm.12 primers selected from 100 ISSR-primers were used for amplification and a total of 148 DNA bands were obtained,including 142 polymorphic bands.The percent of polymorphic is 95.95%.The result showed that the cluster analysis based on ISSR data classified the mango germplasm into 5 major groups.In these groups,most of the samples（87.34%） were gathered in groupⅠ,indicating most of the mango germplasm with close genetic relationship.In this study,all cultivars tested were distinguished from each other and showed ample genetic diversity,indicating that ISSR-PCR was an effective method for cultivar identification of mangoes.