黑龙江八一农垦大学学报
黑龍江八一農墾大學學報
흑룡강팔일농은대학학보
JOURNAL OF HEILONGJIANG AUGUST FIRST LAND RECLAMATION UNIVERSITY
2012年
2期
76-79,83
,共5页
001x7阳离子交换树脂%黄豆酱多肽%脱盐
001x7暘離子交換樹脂%黃豆醬多肽%脫鹽
001x7양리자교환수지%황두장다태%탈염
001×7 cation exchange resin%soybean paste peptide%desalination
为了进行黄豆酱中低聚肽的分离，去除黄豆酱中的盐分。以脱盐率、肽吸附率为指标，对黄豆酱脱盐的吸附树脂种类、动态吸附及解吸条件进行了研究。筛选出了强酸性苯乙烯系阳离子交换树脂001x7为吸附剂，动态吸附的最佳脱盐条件为进样浓度4mg·mL^-l,pH为8的50％乙醇溶液为洗脱剂，流速30r·min^-l,在此条件下，脱盐率为91．28％，肽回收率为88．54％。研究表明，采用001x7阳离子交换树脂对黄豆酱脱盐是一种有效的方法。本试验为黄豆酱多肽的进一步分离、生物活性评价及其一级结构鉴定奠定了研究基础。
為瞭進行黃豆醬中低聚肽的分離，去除黃豆醬中的鹽分。以脫鹽率、肽吸附率為指標，對黃豆醬脫鹽的吸附樹脂種類、動態吸附及解吸條件進行瞭研究。篩選齣瞭彊痠性苯乙烯繫暘離子交換樹脂001x7為吸附劑，動態吸附的最佳脫鹽條件為進樣濃度4mg·mL^-l,pH為8的50％乙醇溶液為洗脫劑，流速30r·min^-l,在此條件下，脫鹽率為91．28％，肽迴收率為88．54％。研究錶明，採用001x7暘離子交換樹脂對黃豆醬脫鹽是一種有效的方法。本試驗為黃豆醬多肽的進一步分離、生物活性評價及其一級結構鑒定奠定瞭研究基礎。
위료진행황두장중저취태적분리，거제황두장중적염분。이탈염솔、태흡부솔위지표，대황두장탈염적흡부수지충류、동태흡부급해흡조건진행료연구。사선출료강산성분을희계양리자교환수지001x7위흡부제，동태흡부적최가탈염조건위진양농도4mg·mL^-l,pH위8적50％을순용액위세탈제，류속30r·min^-l,재차조건하，탈염솔위91．28％，태회수솔위88．54％。연구표명，채용001x7양리자교환수지대황두장탈염시일충유효적방법。본시험위황두장다태적진일보분리、생물활성평개급기일급결구감정전정료연구기출。
Soybean paste was desahed for separating the oligopeptides by using the method of desalination dynamic adsorption and desorption. The desalination rate and peptide adsorption rate were used to analysis the effect of desalination conditions of soybean paste. The results showed that the 001 ×7 strong acid styrene cation exchange resin was appropriate adsorbent, the optimum desalination condition as follows： loading sample concentration was 4 mg＇mL^-1, the pH=8 of 50% alcohol was eluent, the flow speed was 30 r.min^-1. Optimally, the ratio of desalination reached to 91.28%, and the recovery of peptides was 88.54%. This study suggested that desalination method by using 001 ×7 cation exchange resin adsorption resin applied to soybean paste was an available method. This study provided experimental foundation for further research about the separation, activity and the identification primary structure of soybean paste peptides.
