CAJ | 학술논문

根据Genbank发表的牛呼吸道综合征相关病毒中牛传染性鼻气管炎病毒（IBRV ）gB基因、牛呼吸道合胞体病毒（BRSV ）N基因、牛病毒性腹泻病毒（BVDV ）5′-UTR和牛副流感3型病毒（BPIV3）N基因设计特异性引物，建立了在同一体系内同时对4种病毒进行检测的多重PCR方法。该方法可以同时检测出DNA病毒IBRV的长306bp和反转录病毒BPIV3长422bp、BRSV长600bp及BVDV长130bp的特异性片段。本项研究中以已知IBRV、BPIV3、BRSV和BVDV作为参考毒株，对来自内蒙古、辽宁、安徽和吉林4个省、自治区的33例临床样品进行检测。结果表明，合并感染2种病毒较为多见，分别为BVDV和BRSV共感染，BPIV3和BRSV共感染，BPIV3和IBRV共感染，未检测到4种病毒同时感染临床样品。
근거Genbank발표적우호흡도종합정상관병독중우전염성비기관염병독（IBRV ）gB기인、우호흡도합포체병독（BRSV ）N기인、우병독성복사병독（BVDV ）5′-UTR화우부류감3형병독（BPIV3）N기인설계특이성인물，건립료재동일체계내동시대4충병독진행검측적다중PCR방법。해방법가이동시검측출DNA병독IBRV적장306bp화반전록병독BPIV3장422bp、BRSV장600bp급BVDV장130bp적특이성편단。본항연구중이이지IBRV、BPIV3、BRSV화BVDV작위삼고독주，대래자내몽고、료녕、안휘화길림4개성、자치구적33례림상양품진행검측。결과표명，합병감염2충병독교위다견，분별위BVDV화BRSV공감염，BPIV3화BRSV공감염，BPIV3화IBRV공감염，미검측도4충병독동시감염림상양품。
A multiplex PCR(mPCR) assay was developed and evaluated for its ability to detect three viral pathogens including bovine viral diar-rhea virus ,parainfluenza type-3 virus and bovine respiratory syncytial virus associated with the bovine respiratory disease complex .Four pairs of primers were designed ,and each target produced a specific fragment with a size of 130bp(BVDV) ,422bp(BPIV3) ,306bp(IBRV)or 600bp (BRSV ) respectively .In this study ,four well-characterized viral strains BVDV ,BPIV3 ,IBRV and BRSV were used as reference strains .At the same time ,the specificity was confirmed compared to the other bovine viruses .For field application ,among 33 clinical samples from Inner Mon-golia ,Jilin ,Liaoning and Anhui ,co-infection by BVDV and BRSV ,co-infection by BPIV3 and BRSV ,co-infection by BPIV3 and IBRV was tested ,co-infection by those four viruses is not tested .