CAJ | 학술논문

目的：探讨不同类型肝囊型包虫病（CE）患者外周血中白细胞介素-17（IL-17）、白细胞介素-10（IL-10）在疾病发展过程中的作用及对囊肿生物活性判断的价值。方法：将33例CE患者和21例健康体检者按WHO的囊型包虫病B超分类原则共分为4组：健康对照组21例，Ⅰ、Ⅱ型包虫病组14例，Ⅲ型包虫病组10例，Ⅳ、Ⅴ型包虫病组9例。采用酶联免疫吸附法（ELISA法）检测各组IL-17、IL-10的表达。结果：Ⅲ型包虫病组IL-17水平显著高于其他各型包虫病组，差异有统计学意义（P＜0．05）；Ⅰ、Ⅱ型包虫病组IL-17水平[（38．57±3．83） pg/mL]与Ⅳ、Ⅴ型包虫病组[（40．22±5．15） pg/mL]比较无明显差异。Ⅲ型包虫病组IL-10水平[（14．65±2．15） pg/mL]显著高于Ⅰ、Ⅱ型包虫病组[（11．38±0．71） pg/mL]，Ⅰ、Ⅱ型包虫病组IL-10水平显著高于Ⅳ、Ⅴ型包虫病组[（7．60±0．46） pg/mL]，差异有统计学意义（P＜0．05）；Ⅰ、Ⅱ型包虫病组IL-17/IL-10（3．44±1．04）与Ⅲ型包虫病组（4．60±2．40）相比较无明显差异，Ⅲ型包虫病组IL-17/IL-10与Ⅳ、Ⅴ型包虫病组（5．39±1．95）相比较无明显差异，Ⅰ、Ⅱ型包虫病组IL-17/IL-10显著低于Ⅳ、Ⅴ型包虫病组（P＜0．05）。结论：IL-17、IL-10相互协同在促进CE自然病程发展中可能起到一定作用，IL-17和IL-10不能单独作为判断包虫囊肿活性的指标；IL-17/IL-10在一定程度上反应了包虫囊肿的活性，但作为判断各型肝囊型包虫囊肿的活性指标有待进一步讨论验证。
목적：탐토불동류형간낭형포충병（CE）환자외주혈중백세포개소-17（IL-17）、백세포개소-10（IL-10）재질병발전과정중적작용급대낭종생물활성판단적개치。방법：장33례CE환자화21례건강체검자안WHO적낭형포충병B초분류원칙공분위4조：건강대조조21례，Ⅰ、Ⅱ형포충병조14례，Ⅲ형포충병조10례，Ⅳ、Ⅴ형포충병조9례。채용매련면역흡부법（ELISA법）검측각조IL-17、IL-10적표체。결과：Ⅲ형포충병조IL-17수평현저고우기타각형포충병조，차이유통계학의의（P＜0．05）；Ⅰ、Ⅱ형포충병조IL-17수평[（38．57±3．83） pg/mL]여Ⅳ、Ⅴ형포충병조[（40．22±5．15） pg/mL]비교무명현차이。Ⅲ형포충병조IL-10수평[（14．65±2．15） pg/mL]현저고우Ⅰ、Ⅱ형포충병조[（11．38±0．71） pg/mL]，Ⅰ、Ⅱ형포충병조IL-10수평현저고우Ⅳ、Ⅴ형포충병조[（7．60±0．46） pg/mL]，차이유통계학의의（P＜0．05）；Ⅰ、Ⅱ형포충병조IL-17/IL-10（3．44±1．04）여Ⅲ형포충병조（4．60±2．40）상비교무명현차이，Ⅲ형포충병조IL-17/IL-10여Ⅳ、Ⅴ형포충병조（5．39±1．95）상비교무명현차이，Ⅰ、Ⅱ형포충병조IL-17/IL-10현저저우Ⅳ、Ⅴ형포충병조（P＜0．05）。결론：IL-17、IL-10상호협동재촉진CE자연병정발전중가능기도일정작용，IL-17화IL-10불능단독작위판단포충낭종활성적지표；IL-17/IL-10재일정정도상반응료포충낭종적활성，단작위판단각형간낭형포충낭종적활성지표유대진일보토론험증。
Objective To explore the significance of IL-17 and IL-10 in patients with different types of hepatic cystic echinococcosis ( CE ) in cyst stages , to investigate the values of evaluation of hydatid cyst activity . Methods 33 patients with hepatic hydatid disease and 21 healthy people (control group) were enrolled, divided into 4 groups, according to WHO ultrasonic classification for CE:Control group (n=21), CEⅠ,Ⅱgroup (active, n=14), CEⅢgroup (transitional, n=10), and CEⅣ,Ⅴgroup (inactive, n=9). The serum concentrations of IL-17 and IL-10 were measured by enzyme-linked immunosorbent assay. Results Serum levels of IL-17 in CEⅢgroup were significantly higher than other groups (P<0.05). There were no significant difference in serum IL-17 levels between CEⅠ,Ⅱgroup [(38.57 ± 3.83) pg/mL] and CEⅣ,Ⅴgroup [(40.22 ± 5.15) pg/mL]. Serum levels of IL-10 were significantly higher in CEⅢgroup [(14.65 ± 2.15) pg/mL] than CEⅠ,Ⅱgroup [(11.38 ± 0.71) pg/mL] (P<0.05), while CEⅠ,Ⅱgroup were significantly higher than CEⅣ,Ⅴgroup [(7.60 ± 0.46) pg/mL] (P<0.05). There were no significant difference of IL-17/IL-10 ratio between CEⅠ, Ⅱgroup (3.44 ± 1.04) and CEⅢgroup (4.60 ± 2.40), and there were no significant difference of IL-17/IL-10 ratio between CEⅢgroup and CEⅣ,Ⅴgroup (5.39 ± 1.95). IL-17/IL-10 ratio in CEⅠ, Ⅱgroup was significantly lower than CEⅣ, Ⅴgroup (P<0.05). The serum concentrations of IL-17 has no correlation with IL-10 in different cyst stages. Conclusions The synergy effect of IL-17 and IL-10 may play the certain role to promote the parasite progress. Neither IL-17 nor IL-10 level could be as an independent marker of echinococcal cysts activity, while IL-17/IL-10 ratio reflect the hydatid cyst activity in certain degree, but as a echinococcal cysts activity marker, further evidence should be needed.