实用医学杂志
實用醫學雜誌
실용의학잡지
THE JOURNAL OF PRACTICAL MEDICINE
2014年
5期
680-683
,共4页
于莉娜%张庆玲%肖雨%丁彦青
于莉娜%張慶玲%肖雨%丁彥青
우리나%장경령%초우%정언청
结直肠肿瘤%Tiam1%HT29
結直腸腫瘤%Tiam1%HT29
결직장종류%Tiam1%HT29
Colorectal neoplasms%Tiam1%HT29
目的：应用已构建稳定过表达T淋巴瘤侵袭转移诱导因子1（T lymphoma invasion and metastasis， Tiam1）的人结直肠癌细胞株，探讨Tiam1对人结直肠癌细胞生物学特性的影响。方法：考马斯亮蓝染色以及扫描电镜观察Tiam1基因转染前后细胞形态学改变；细胞周期、MTT法、平板克隆形成实验、Transwell小室检测细胞体外增殖、迁移及侵袭能力；裸鼠皮下成瘤实验检测细胞体内增殖能力。结果：相对于HT29/mock细胞，HT29/Tiam1细胞多呈梭形，伪足增多、纤长；HT29/Tiam1细胞处于S期的细胞比例更高（t＝19．546， P＝0．000），细胞增殖能力增强（F＝177．125，P＝0．000），克隆形成率增高（t＝3．222，P＝0．032），HT29/Tiam1细胞穿过微孔膜（t＝4．832，P＝0．001）和Matrigel（t＝3．779，P＝0．005）的细胞数均增加，差异具有显著性；HT29/Tiam1和HT29/mock两组细胞的裸鼠体内生长差异从第15天开始出现，到第30天时，HT29/Tiam1细胞组的肿瘤体积是HT29/mock细胞组的2．3倍，差异具有统计学意义（F＝53．040，P＝0．002）。结论：Tiam1稳定过表达能够促进结直肠癌细胞的增殖、运动及侵袭能力，提示Tiam1在结直肠癌的发生、发展中发挥着重要作用，有望为结直肠癌的临床治疗提供新的靶点。
目的：應用已構建穩定過錶達T淋巴瘤侵襲轉移誘導因子1（T lymphoma invasion and metastasis， Tiam1）的人結直腸癌細胞株，探討Tiam1對人結直腸癌細胞生物學特性的影響。方法：攷馬斯亮藍染色以及掃描電鏡觀察Tiam1基因轉染前後細胞形態學改變；細胞週期、MTT法、平闆剋隆形成實驗、Transwell小室檢測細胞體外增殖、遷移及侵襲能力；裸鼠皮下成瘤實驗檢測細胞體內增殖能力。結果：相對于HT29/mock細胞，HT29/Tiam1細胞多呈梭形，偽足增多、纖長；HT29/Tiam1細胞處于S期的細胞比例更高（t＝19．546， P＝0．000），細胞增殖能力增彊（F＝177．125，P＝0．000），剋隆形成率增高（t＝3．222，P＝0．032），HT29/Tiam1細胞穿過微孔膜（t＝4．832，P＝0．001）和Matrigel（t＝3．779，P＝0．005）的細胞數均增加，差異具有顯著性；HT29/Tiam1和HT29/mock兩組細胞的裸鼠體內生長差異從第15天開始齣現，到第30天時，HT29/Tiam1細胞組的腫瘤體積是HT29/mock細胞組的2．3倍，差異具有統計學意義（F＝53．040，P＝0．002）。結論：Tiam1穩定過錶達能夠促進結直腸癌細胞的增殖、運動及侵襲能力，提示Tiam1在結直腸癌的髮生、髮展中髮揮著重要作用，有望為結直腸癌的臨床治療提供新的靶點。
목적：응용이구건은정과표체T림파류침습전이유도인자1（T lymphoma invasion and metastasis， Tiam1）적인결직장암세포주，탐토Tiam1대인결직장암세포생물학특성적영향。방법：고마사량람염색이급소묘전경관찰Tiam1기인전염전후세포형태학개변；세포주기、MTT법、평판극륭형성실험、Transwell소실검측세포체외증식、천이급침습능력；라서피하성류실험검측세포체내증식능력。결과：상대우HT29/mock세포，HT29/Tiam1세포다정사형，위족증다、섬장；HT29/Tiam1세포처우S기적세포비례경고（t＝19．546， P＝0．000），세포증식능력증강（F＝177．125，P＝0．000），극륭형성솔증고（t＝3．222，P＝0．032），HT29/Tiam1세포천과미공막（t＝4．832，P＝0．001）화Matrigel（t＝3．779，P＝0．005）적세포수균증가，차이구유현저성；HT29/Tiam1화HT29/mock량조세포적라서체내생장차이종제15천개시출현，도제30천시，HT29/Tiam1세포조적종류체적시HT29/mock세포조적2．3배，차이구유통계학의의（F＝53．040，P＝0．002）。결론：Tiam1은정과표체능구촉진결직장암세포적증식、운동급침습능력，제시Tiam1재결직장암적발생、발전중발휘착중요작용，유망위결직장암적림상치료제공신적파점。
Objective To explore the effect of Tiam1 overexpression on the biological behaviors of human colorectal cancer cells ( CRC ) . Methods The human CRC lines under the established stable overexpression of Tiam1 were studied. Cell morphology was detected before and after transfection by commassie blue staining and scanning electron microscope. The proliferation in vitro of CRC was tested by cell cycle, MTT and plate colony formation assay, the migration and invasion ability of CRC was tested by Transwell assay. The proliferation ability in vivo was studied by induced subcutaneous tumors of nude mice. Results Compared with HT29/mock cells, HT29/Tiam1 cells formed as spindle, the pseudopodia increased and elongated. The proportion in S phase of HT29/Tiam1 was higher (t=19.546, P=0.000), the proliferation ability enhanced (F=177.125, P=0.000), colonies formation ratio increased ( t = 3 . 222 , P = 0 . 032 ) . The number of HT29/Tiam1 cells acrossing the microporous membrane (t = 4.832, P=0.001)and Matrigel(t=3.779, P = 0.005)all raised. On the fifteenth day, the growth deference between the HT29/Tiam1cells and HT29/mock cells in nude mice in vivo occurred. Till the thirtieth day, the size of the tumors in HT29/Tiam1 cell group were 2.3 times as large as that in HT29/mock cell group (F=53.040, P=0.002). Conclusions Tiam1 stable overexpression can promote the proliferation, migration and invasion ability of CRC which indicates its important role in carcinogenesis and evolution of colorectal cancer. Tiam1 may represent a new therapeutic target for colorectal cancer.
