中国动物检疫
中國動物檢疫
중국동물검역
CHINA ANMAL QUARANTINE
2012年
5期
42-45
,共4页
繁殖障碍综合征%病因研究%猪
繁殖障礙綜閤徵%病因研究%豬
번식장애종합정%병인연구%저
Reproductive disorder syndrome%Etiological studies%Pig
针对红河州猪繁殖障碍综合征危害日趋严重的现象，进行了该病的流行病学、病原学、人工感染及免疫试验。结果表明，种公猪发病率为23．77％、妊娠母猪发病率为41．38％，仔猪死亡率为19．94％、流产率11．19％、死胎率47．96％、弱仔率31．78％；血清学调查阳性检出率分别为猪瘟（CSF）1．1％、细小病毒（PPV）43．97％、鹦鹉热衣原体（CP）36．98％、伪狂犬病毒（PRV）24．6％、乙型脑炎（JEV）20．9％、弓形虫（Tg）14．66％、蓝耳病病毒（PRRSV）11．5％、猪圆环病毒（PCV）7．2％、布鲁氏菌（Br）2．44％。其中单一感染占39．6％，混合感染占35．6％。从死亡新生仔猪和流产胎儿的脑及内脏中分离获得PPV、PRV、CP各2株病原，经细胞传代，鸡胚传代，毒价测定、形态观察、动物感染试验和应用PCR技术等鉴定，确证为伪狂犬、细小病毒病、衣原体三种。应用猪伪狂犬、细小病毒病、衣原体三种疫苗免疫注射，使本地区猪群的受胎率由92．7％提高到99．5‰妊娠母猪发病率从31．43％下降到0．77％，初生乳猪发病死亡率从24．9％下降到0．27％。调查研究表明，近年来引起红河州地区猪繁殖障碍综合征广泛流行的主要病因是PPV、PRV、CP三种病原单一或混合感染所致，而且动物感染试验证实是PPV、PRV、CP是造成猪繁殖障碍病的主要病原。
針對紅河州豬繁殖障礙綜閤徵危害日趨嚴重的現象，進行瞭該病的流行病學、病原學、人工感染及免疫試驗。結果錶明，種公豬髮病率為23．77％、妊娠母豬髮病率為41．38％，仔豬死亡率為19．94％、流產率11．19％、死胎率47．96％、弱仔率31．78％；血清學調查暘性檢齣率分彆為豬瘟（CSF）1．1％、細小病毒（PPV）43．97％、鸚鵡熱衣原體（CP）36．98％、偽狂犬病毒（PRV）24．6％、乙型腦炎（JEV）20．9％、弓形蟲（Tg）14．66％、藍耳病病毒（PRRSV）11．5％、豬圓環病毒（PCV）7．2％、佈魯氏菌（Br）2．44％。其中單一感染佔39．6％，混閤感染佔35．6％。從死亡新生仔豬和流產胎兒的腦及內髒中分離穫得PPV、PRV、CP各2株病原，經細胞傳代，鷄胚傳代，毒價測定、形態觀察、動物感染試驗和應用PCR技術等鑒定，確證為偽狂犬、細小病毒病、衣原體三種。應用豬偽狂犬、細小病毒病、衣原體三種疫苗免疫註射，使本地區豬群的受胎率由92．7％提高到99．5‰妊娠母豬髮病率從31．43％下降到0．77％，初生乳豬髮病死亡率從24．9％下降到0．27％。調查研究錶明，近年來引起紅河州地區豬繁殖障礙綜閤徵廣汎流行的主要病因是PPV、PRV、CP三種病原單一或混閤感染所緻，而且動物感染試驗證實是PPV、PRV、CP是造成豬繁殖障礙病的主要病原。
침대홍하주저번식장애종합정위해일추엄중적현상，진행료해병적류행병학、병원학、인공감염급면역시험。결과표명，충공저발병솔위23．77％、임신모저발병솔위41．38％，자저사망솔위19．94％、유산솔11．19％、사태솔47．96％、약자솔31．78％；혈청학조사양성검출솔분별위저온（CSF）1．1％、세소병독（PPV）43．97％、앵무열의원체（CP）36．98％、위광견병독（PRV）24．6％、을형뇌염（JEV）20．9％、궁형충（Tg）14．66％、람이병병독（PRRSV）11．5％、저원배병독（PCV）7．2％、포로씨균（Br）2．44％。기중단일감염점39．6％，혼합감염점35．6％。종사망신생자저화유산태인적뇌급내장중분리획득PPV、PRV、CP각2주병원，경세포전대，계배전대，독개측정、형태관찰、동물감염시험화응용PCR기술등감정，학증위위광견、세소병독병、의원체삼충。응용저위광견、세소병독병、의원체삼충역묘면역주사，사본지구저군적수태솔유92．7％제고도99．5‰임신모저발병솔종31．43％하강도0．77％，초생유저발병사망솔종24．9％하강도0．27％。조사연구표명，근년래인기홍하주지구저번식장애종합정엄범류행적주요병인시PPV、PRV、CP삼충병원단일혹혼합감염소치，이차동물감염시험증실시PPV、PRV、CP시조성저번식장애병적주요병원。
The epidemiological investigations, etiological tests, artifitial infection tests and immunization tests were carried out in order to reveal the causes for serious prevalence of reproductive disorder syndrome in pigs recently in Honghe region of Yunnan province. The results showed that the morbidity was 23.77% for breeding boars, for 41.38% pregnant sows, and the piglet mortality in perinatal period reached 19.94%, the abortion rate of infected pregnant sows was 11.19%, stillbirth 47.96%, weak babies 31.78%. Specimens were sampled from pig herds which had not been inoculated with any vaccines for serologic investigations, resulting in classical swine fever virus （CSF） 1.1%, porcine parvovirus （PPV） 43.97%, Chlamydia psittaci （CP） 36.98%, pseudorabies virus （PRV） 24.6%, Japanese encephalitis virus （JEV） 20.9%, Toxoplasma gondii （Tg） 14.66%, porcine reproductive and respiratory syndrome virus （PRRSV） 11.5%, porcine circovirus （PCV） 7.2%, and Brucella sp （Br） 2.44%, with 39.6% single agent infection and 35.6% mixed infection.Two strains each of PPV, CP and PRV were isolated from died newborn piglets and aborted fetus respectively, and detected and identified by means of PCR. The laboratory animals were artificially infected with the isolated agents resulting in same symptoms as the field cases.After pig herds in this region were inoculated with PPV vaccine, CP vaccine and PRV vaccine respectively, the pregnant rate was increased from 92.7% to 99.5%, the morbidly of piglets was reduced from 31.43% to 0.77%, and the mortality of piglets was reduced from 24.9% to 0.27%. The study confirmed that porcine parvovirus, Chlamydia psittaci and pseudorabies virus were the major pathogens causing swine reproductive disorder syndromes by means of single or multiple infections.
