宜春学院学报
宜春學院學報
의춘학원학보
JOURNAL OF YICHUN UNIVERSITY
2012年
4期
106-107,119
,共3页
海藻多糖%动物模型%SGPT%LPO%抗氧化
海藻多糖%動物模型%SGPT%LPO%抗氧化
해조다당%동물모형%SGPT%LPO%항양화
PSP%animal models%SGPT%LPO%anti-oxidation
目的：研究海藻多糖（Polysaechrides from Spirulina Platensis,PSP）对小鼠肝损伤动物模型与肝匀浆脂质过氧化模型的抗氧化作用。方法：采用腹腔注射CCL4的方法,制造小鼠肝损伤动物模型,金氏法测定血清谷氨酸丙酮酸转氨酶（SGPT）和肝脏的过氧化脂质（Lipoper-oxides,LPO）含量;Fenton反应诱导小鼠肝匀浆脂质过氧化动物模型,采用硫代巴比妥酸（TBA）法测定肝匀浆LPO。结果：在体外,海藻多糖可剂量依赖性地抑制Fenton反应导致的CCL4毒化小鼠肝匀浆中LPO的生成,其IC50为1.6×10-4。在体内,灌胃250mg/kg的海藻多糖组可明显降低CCL4毒化小鼠血清SGPT活性（P〈0.01）。结论：海藻多糖对体内外的肝组织有一定程度的抗氧化作用。
目的：研究海藻多糖（Polysaechrides from Spirulina Platensis,PSP）對小鼠肝損傷動物模型與肝勻漿脂質過氧化模型的抗氧化作用。方法：採用腹腔註射CCL4的方法,製造小鼠肝損傷動物模型,金氏法測定血清穀氨痠丙酮痠轉氨酶（SGPT）和肝髒的過氧化脂質（Lipoper-oxides,LPO）含量;Fenton反應誘導小鼠肝勻漿脂質過氧化動物模型,採用硫代巴比妥痠（TBA）法測定肝勻漿LPO。結果：在體外,海藻多糖可劑量依賴性地抑製Fenton反應導緻的CCL4毒化小鼠肝勻漿中LPO的生成,其IC50為1.6×10-4。在體內,灌胃250mg/kg的海藻多糖組可明顯降低CCL4毒化小鼠血清SGPT活性（P〈0.01）。結論：海藻多糖對體內外的肝組織有一定程度的抗氧化作用。
목적：연구해조다당（Polysaechrides from Spirulina Platensis,PSP）대소서간손상동물모형여간균장지질과양화모형적항양화작용。방법：채용복강주사CCL4적방법,제조소서간손상동물모형,금씨법측정혈청곡안산병동산전안매（SGPT）화간장적과양화지질（Lipoper-oxides,LPO）함량;Fenton반응유도소서간균장지질과양화동물모형,채용류대파비타산（TBA）법측정간균장LPO。결과：재체외,해조다당가제량의뢰성지억제Fenton반응도치적CCL4독화소서간균장중LPO적생성,기IC50위1.6×10-4。재체내,관위250mg/kg적해조다당조가명현강저CCL4독화소서혈청SGPT활성（P〈0.01）。결론：해조다당대체내외적간조직유일정정도적항양화작용。
Objective To study the antioxidant effects of the seaweed polysaccharides（Polysaechrides from Spirulina Platensis,PSP） on liver injury in animal models and liver homogenate lipid peroxidation models.Methods The Intraperitoneal injection of CCL was conducted to create animal models of liver injury in mice;Kim method was taken to measure the content of serum glutamate pyruvate transaminase（SGPT） and liver lipid peroxidation（Lipoperoxides,LPO）;Fenton reaction induced mouse liver homogenate lipid peroxidation in animal models.The thiobarbituric acid（TBA） method was adopted for the determination of liver homogenate LPO.Results In vitro,seaweed polysaccharides dose-dependently inhibited LPO generation in mice liver homogenate poisoned by CCL4,which was resulted from the Fenton reaction.IC50= 1.6×10-4.In vivo,the fed 250mg/kg of seaweed polysaccharide group can significantly reduce the SGPT activity of CCL4 in poisoning the mouse serum（P0.01）.Conclusion Seaweed polysaccharides has a certain degree of anti-oxidation on liver tissue in vitro and in vivo.
