CAJ | 학술논문

本文采用毛细管区带电泳-安培检测法（CZE-AD）,研究了四类菊花中的常见单糖及蔗糖的分离、检测方法。在选定的实验条件下,可在20分钟内实现对葡萄糖、果糖、半乳糖、蔗糖的有效分离;待测糖类在铜电极上具有良好的电流响应、检测灵敏度（LOD：～1.0×10-6mol/L）及适宜的线性范围（5.0×10-6～5.0×10-4mol/L）。实验对滁菊、杭黄菊、贡菊、亳菊中的糖类组分进行了CZE-AD分离检测,方法具有良好的重现性及应用前景。检测结果表明,四类菊花中常见糖类组分的含量差异较大,可为相关植物资源的开发利用提供参考;检测方法简单、快速、有效,可用于菊花中常见糖类成分的测定及质量控制。
본문채용모세관구대전영-안배검측법（CZE-AD）,연구료사류국화중적상견단당급자당적분리、검측방법。재선정적실험조건하,가재20분종내실현대포도당、과당、반유당、자당적유효분리;대측당류재동전겁상구유량호적전류향응、검측령민도（LOD：～1.0×10-6mol/L）급괄의적선성범위（5.0×10-6～5.0×10-4mol/L）。실험대저국、항황국、공국、박국중적당류조분진행료CZE-AD분리검측,방법구유량호적중현성급응용전경。검측결과표명,사류국화중상견당류조분적함량차이교대,가위상관식물자원적개발이용제공삼고;검측방법간단、쾌속、유효,가용우국화중상견당류성분적측정급질량공제。
In this paper,the determination of normal saccharides in four kinds of Chrysanthemi Flos was investigated with capillary zone electrophoresis-amperometric detection method（CZE-AD）.Under the optimized conditions,glucose,fructose,galactose and sucrose could be separated in 20 min.These analyzed saccharides gained excellent current response,detection limits（LOD）： ~1.0×10-6mol/L and adequate linear ranges（5.0×10-6~5.0×10-4mol/L）.The saccharides in four kinds of Chrysanthemi Flos（Chu-Ju,Hanghuang-Ju,Gong-Ju,Bo-Ju） were analyzed,and the results indicated good reproducibility and applicability of this proposed CZE-AD method.It could be found that the concerns of saccharides varied from one kind of Chrysanthemi Flos to another,and the detection data would provide reference to exploit the corresponding plant resources efficiently.This proposed detection method was simple,quick and effective with potential application in the analysis of saccharides for quality control of Chrysanthemi Flos.