CAJ | 학술논문

目的：通过两种不同方法大量分离乳猪肝细胞，观察分离肝细胞的活性和功能，探讨猪肝细胞的分离方法。方法：本实验以肝上下腔静脉作为体外乳肝细胞分离的灌注口，用倒置相差显微镜、血细胞计数板计数，台盼蓝拒染试验判断细胞活力，并用自动生化仪进行肝细胞功能的检测。结果：实验组分离的每克肝肝细胞数和肝细胞活性明显高于对照组，两者差异有统计学意义（P＜0.05）；实验组AST、ALT、LDH以及TBIL浓度低于对照组，但差异无统计学意义（P＞0.05）。结论：本实验改进了胶原酶体外两步灌流法分离乳猪肝细胞，为肝细胞的大量分离提供了一定的理论和实验依据。
목적：통과량충불동방법대량분리유저간세포，관찰분리간세포적활성화공능，탐토저간세포적분리방법。방법：본실험이간상하강정맥작위체외유간세포분리적관주구，용도치상차현미경、혈세포계수판계수，태반람거염시험판단세포활력，병용자동생화의진행간세포공능적검측。결과：실험조분리적매극간간세포수화간세포활성명현고우대조조，량자차이유통계학의의（P＜0.05）；실험조AST、ALT、LDH이급TBIL농도저우대조조，단차이무통계학의의（P＞0.05）。결론：본실험개진료효원매체외량보관류법분리유저간세포，위간세포적대량분리제공료일정적이론화실험의거。
Objective To study the method of primary porcine hepatocyte isolation by observe the hepatocyte quantity、viability and function.Methods Porcine hepatocytes were isolated by two-step perfusion through the supra-hepatic inferior vena cava,counting the hepatocyte quantity,observing the hepatocyte viability,and testing the levels of AST、ALT、LDH、TBIL.Results There were significant difference in hepatocyte viability and hepatocyte quantity per gram between two groups(P<0.05),there were no significant difference in the concentration average of AST、ALT、LDH and TBIL between two groups(P>0.05).Conclusion The experiment improved the method of porcine hepatocyte isolation,and provided some theoretical and experimental evidence.