中国食物与营养
中國食物與營養
중국식물여영양
Food and Nutrition in China
2012年
4期
59-63
,共5页
浒苔多糖%脱蛋白%脱盐%酶法%透析法
滸苔多糖%脫蛋白%脫鹽%酶法%透析法
호태다당%탈단백%탈염%매법%투석법
enteromorpha polysaccharides%de-protein%desalting%enzymatic method%dialysis method
目的：探讨浒苔多糖脱蛋白脱盐的方法。方法：利用蛋白酶水解浒苔多糖的蛋白质,通过正交试验优选蛋白酶水解工艺条件;利用透析袋流水透析脱盐,通过正交试验优选透析工艺条件。结果：中性蛋白酶脱蛋白效果优于Sevag法和三氯乙酸法,中性蛋白酶脱蛋白较佳工艺条件为：加酶量0.12g/10mL、pH6.0、反应温度45℃、反应时间1h,在此条件下,蛋白质脱除率达52.36%。透析脱盐的较佳条件为：7000Da、透析时间8h、浒苔多糖溶液浓度6%,在此条件下,灰分脱除率为76.95%。（除杂后,浒苔多糖蛋白质含量为3.43%,灰分含量为8.04%,多糖损失率为18.96%）。结论：蛋白酶法脱蛋白质技术和透析袋流水透析脱盐技术,对浒苔多糖的脱蛋白脱盐效果较好,有一定的推广潜力。
目的：探討滸苔多糖脫蛋白脫鹽的方法。方法：利用蛋白酶水解滸苔多糖的蛋白質,通過正交試驗優選蛋白酶水解工藝條件;利用透析袋流水透析脫鹽,通過正交試驗優選透析工藝條件。結果：中性蛋白酶脫蛋白效果優于Sevag法和三氯乙痠法,中性蛋白酶脫蛋白較佳工藝條件為：加酶量0.12g/10mL、pH6.0、反應溫度45℃、反應時間1h,在此條件下,蛋白質脫除率達52.36%。透析脫鹽的較佳條件為：7000Da、透析時間8h、滸苔多糖溶液濃度6%,在此條件下,灰分脫除率為76.95%。（除雜後,滸苔多糖蛋白質含量為3.43%,灰分含量為8.04%,多糖損失率為18.96%）。結論：蛋白酶法脫蛋白質技術和透析袋流水透析脫鹽技術,對滸苔多糖的脫蛋白脫鹽效果較好,有一定的推廣潛力。
목적：탐토호태다당탈단백탈염적방법。방법：이용단백매수해호태다당적단백질,통과정교시험우선단백매수해공예조건;이용투석대류수투석탈염,통과정교시험우선투석공예조건。결과：중성단백매탈단백효과우우Sevag법화삼록을산법,중성단백매탈단백교가공예조건위：가매량0.12g/10mL、pH6.0、반응온도45℃、반응시간1h,재차조건하,단백질탈제솔체52.36%。투석탈염적교가조건위：7000Da、투석시간8h、호태다당용액농도6%,재차조건하,회분탈제솔위76.95%。（제잡후,호태다당단백질함량위3.43%,회분함량위8.04%,다당손실솔위18.96%）。결론：단백매법탈단백질기술화투석대류수투석탈염기술,대호태다당적탈단백탈염효과교호,유일정적추엄잠력。
【Objective】 To study the method of de-protein and desalting process for enteromorpha polysaccharides.【Method】The best method of enzymatic de-protein process was screened from amino-acid nitrogen.The proper method of run water dialysis was chosen from dry ash content and polysaccharides loss rate.【Result】De-protein effect of neutral protease was better than that of papain method,Sevag method and trichloroacetic acid method.The best condition of neutral protease de-protein process was adding 0.12g/10mL of lipase amounts into the extracting liquid,adjusting the pH to 6.0,then keeping it in the constant temperature bath 45℃ for 1h.The proper method of run water dialysis was keeping 6% enteromorpha extracting liquid in the 7？000Da dialysis bag for 8h.The protein content of purer enteromorpha polysaccharides was 3.43%,ash content was 8.04%,and polysaccharides loss rate was 18.96%.【Conclusion】 Neutral protease method and of run water dialysis method are the better ways for de-protein effect and desalting process,which can be used universally.
