大理学院学报：综合版
大理學院學報：綜閤版
대이학원학보：종합판
Journal of Dali University
2012年
3期
31-34
,共4页
倪兆林%申元英%白丽%陈红云%李润兵
倪兆林%申元英%白麗%陳紅雲%李潤兵
예조림%신원영%백려%진홍운%리윤병
黑木耳%乙醇浸提法%紫外分光光度苯酚-硫酸显色法%多糖%含量
黑木耳%乙醇浸提法%紫外分光光度苯酚-硫痠顯色法%多糖%含量
흑목이%을순침제법%자외분광광도분분-류산현색법%다당%함량
Auricularia auricula%ethanol extraction%UV spectrophotometry phenol-sulfuric acid method%polysaccharides%content
目的：提取云龙野生和人工培养两种黑木耳多糖并测定其含量。方法：采用乙醇浸提法提取多糖,紫外分光光度苯酚-硫酸显色法测定粗多糖含量。结果：在8.0-48.0μg/mL线性范围内,回收率为99.03%,RSD为0.66%（n=6）,测到野生和人工培养黑木耳中多糖的收率分别为18.48%和18.29%;野生和人工培养黑木耳中多糖的含量分别为9.21%和8.20%。结论：野生黑木耳比人工培养黑木耳中多糖的含量高,生长环境对黑木耳多糖含量有影响。
目的：提取雲龍野生和人工培養兩種黑木耳多糖併測定其含量。方法：採用乙醇浸提法提取多糖,紫外分光光度苯酚-硫痠顯色法測定粗多糖含量。結果：在8.0-48.0μg/mL線性範圍內,迴收率為99.03%,RSD為0.66%（n=6）,測到野生和人工培養黑木耳中多糖的收率分彆為18.48%和18.29%;野生和人工培養黑木耳中多糖的含量分彆為9.21%和8.20%。結論：野生黑木耳比人工培養黑木耳中多糖的含量高,生長環境對黑木耳多糖含量有影響。
목적：제취운룡야생화인공배양량충흑목이다당병측정기함량。방법：채용을순침제법제취다당,자외분광광도분분-류산현색법측정조다당함량。결과：재8.0-48.0μg/mL선성범위내,회수솔위99.03%,RSD위0.66%（n=6）,측도야생화인공배양흑목이중다당적수솔분별위18.48%화18.29%;야생화인공배양흑목이중다당적함량분별위9.21%화8.20%。결론：야생흑목이비인공배양흑목이중다당적함량고,생장배경대흑목이다당함량유영향。
Objective： To extract both artificial culture and wild Auricularia auricular polysaccharides and determinate their contents.Methods： The polysaccharide was extracted with ethanol and the polysaccharide content was wired by UV spectrophotometry phenol-sulfuric acid color method.Results： Within the range of 8.0-48.0 μg/mL,the recovery ratio of polysaccharide was 99.03% with RDS of 0.66%（n=6）.The recovery ratio of polysaccharide in artificial culture and wild Auricularia auricular were 18.48% and 18.29%,respectively.Artificial culture and wild Auricularia auricular in the polysaccharide content was 9.21% and 8.20%,respectively.Conclusion： Wild Auricularia auricular has higher content of polysaccharides than that of culture artificially.Growing environment affects the contents of Auricularia auricula＇s polysaccharides.
