CAJ | 학술논문

目的：克隆福氏志贺菌（Shigella flexneri,S.flexne）的ipaB基因。方法：以福氏志贺菌2a 2457T（Nal）r为模板,用PCR扩增ipaB目的基因片段,克隆至pQE-30表达载体中,构建含目的基因的表达质粒pQE-ipaB。结果：构建了重组质粒pQE-ipaB,ipaB基因全长1 743 bp,经测序分析与预期相符。结论：成功克隆了ipaB基因。
목적：극륭복씨지하균（Shigella flexneri,S.flexne）적ipaB기인。방법：이복씨지하균2a 2457T（Nal）r위모판,용PCR확증ipaB목적기인편단,극륭지pQE-30표체재체중,구건함목적기인적표체질립pQE-ipaB。결과：구건료중조질립pQE-ipaB,ipaB기인전장1 743 bp,경측서분석여예기상부。결론：성공극륭료ipaB기인。
Objective：To clone and sequence of ipaB gene of Shigella flexneri.Methods：IpaB gene was amplified by PCR.The DNA products of ipaB were inserted into a prokaryotic expression vector pQE-30,and then translated into E.coli strain Top10,restriction digestion and sequencing.Results：The recombinant plasmid was constructed and ipaB gene was sequenced as 1 743 bp,conformity with expectation.Conclusion：The results indicated that recombinant plasmid was constructed successfully.