CAJ | 학술논문

目的：观察幼鼠小肠缺血-再灌注（ischemia/reperfusion,I/R）肠组织形态学变化。方法：将32只4周龄（Sprague Dawley,SD）大鼠随机分成2组即假手术组（Sham组）和缺血-再灌注组（I/R组）,每组各16只。Sham组仅暴露腹腔脏器,不作任何干预;I/R组经阻断肠系膜上动脉60 min后恢复血供,于再灌注30、60、90、120 min每组动物各处死4只取小肠组织进行组织学Chiu’s评分,结果进行单因素方差分析和组间t检验。结果：Sham组肠组织形态正常;I/R组小肠组织发生了明显的缺血和炎症反应并随再灌注时间延长而加重,各观察时间点组织学评分在组间比较差异均有统计学意义,各值在I/R组高于Sham组（P〈0.05）。结论：幼鼠肠I/R导致肠组织病理损伤并随再灌注时间延长而加重。
목적：관찰유서소장결혈-재관주（ischemia/reperfusion,I/R）장조직형태학변화。방법：장32지4주령（Sprague Dawley,SD）대서수궤분성2조즉가수술조（Sham조）화결혈-재관주조（I/R조）,매조각16지。Sham조부폭로복강장기,불작임하간예;I/R조경조단장계막상동맥60 min후회복혈공,우재관주30、60、90、120 min매조동물각처사4지취소장조직진행조직학Chiu’s평분,결과진행단인소방차분석화조간t검험。결과：Sham조장조직형태정상;I/R조소장조직발생료명현적결혈화염증반응병수재관주시간연장이가중,각관찰시간점조직학평분재조간비교차이균유통계학의의,각치재I/R조고우Sham조（P〈0.05）。결론：유서장I/R도치장조직병리손상병수재관주시간연장이가중。
Objective： To explore the intestinal histological changes of intestinal ischemic/reperfusion（I/R） injury in young rats.Methods： 32 Sprague Dawley（SD） rats at 4 weeks old were divided into 2 groups with 16 in each group randomly,and there were sham operated group as a control and I/R group.The abdominal cavity were exposed in all rats but not any intervene in sham group,the superior mesenteric artery were occluded in I/R group for 60 min and followed by 30 min,60 min,90 min and 120 min for reperfusion and 4 rats were harvested at each time point.The intestine specimens were obtained for pathological study and determination of histopathologic score.The data were analyzed statistically.Results： There were pathological changes of ischemia and inflammatory obviously and getting severer with the reperfusion time prolong in all rats but in the sham group.There was statistical significance as a result of the Chiu＇s score between two groups in every time point（P〈0.05）.The scores in I/R group were higher than that in sham group（P〈0.05）.The score was highest at 90min among four time points in each I/R group.Conclusion： The pathological changes of intestinal injury caused by I/R were getting severer with reperfusion prolong.