CAJ | 학술논문

马链球菌兽疫亚种是引起猪链球菌病的主要病原之一。该菌的致病机理尚不清楚，且缺乏合适的疫苗，使猪链球菌病很难得到有效的控制。本实验通过构建SzP重组表达载体，纯化重组蛋白，对其的免疫效力进行了评价。结果表明该蛋白可以诱导高滴度的血清IgG抗体，并且可提供一定的免疫保护效力。进一步研究表明该蛋白是一个重要的体内诱导抗原，可诱导高水平的Thl和Th2型免疫应答。揭示了SzP蛋白在致病过程中起到重要作用，为新型疫苗的研制及致病机制的研究奠定了基础。
마련구균수역아충시인기저련구균병적주요병원지일。해균적치병궤리상불청초，차결핍합괄적역묘，사저련구균병흔난득도유효적공제。본실험통과구건SzP중조표체재체，순화중조단백，대기적면역효력진행료평개。결과표명해단백가이유도고적도적혈청IgG항체，병차가제공일정적면역보호효력。진일보연구표명해단백시일개중요적체내유도항원，가유도고수평적Thl화Th2형면역응답。게시료SzP단백재치병과정중기도중요작용，위신형역묘적연제급치병궤제적연구전정료기출。
Streptococcus equi ssp. zooepidemicus （SEZ） is an important pathogen associated with opportunistic infections of pigs in China. The absence of suitable vaccines or the knowledge of virulence markers could block the step for controlling SEZ infection. M-like protein （SzP） has been identified as an immunogenic protein in the previous study but its protective efficacy was not clear. In the present study, the purified recombinant SzP could elicit a significant humoral antibody response and could confer significant protection against challenge with lethal dose of SEZ in mice model. In addition, the SzP was an in vivo-induced antigen confirmed by the real-time PCR and could induce significant Thl-/Th2-immune responses. Our findings suggested that SzP played an important role in the pathogenesis of SEZ and could be a target for the development of an effective vaccine against SEZ infection.